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相关概念视频

CRISPR01:59

CRISPR

50.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jun 21, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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为CRISPR/Cas辅助生物感知而设计的crRNA

Long Ma1, Minghui Lu1, Jingyu Jia1

  • 1Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin, 300457, China; Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin, 300457, China; China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin, 300457, China.

Trends in biotechnology
|July 9, 2024
PubMed
概括

工程CRISPRRNA (crRNA) 的进步克服了基于CRISPR的诊断 (CRISPR-Dx) 的局限性. 这些创新改进了短核酸和非核酸点的检测,提高了诊断能力.

关键词:
这就是CRISPR/CasPR.挑战和机遇 挑战和机遇检测,CRISPR-Dx的使用方法.通过工程设计的crRNA.

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 诊断 诊断 诊断 诊断

背景情况:

  • 基于CRISPR/Cas的诊断 (CRISPR-Dx) 提供了有前途的分子检测,但面临着重大挑战.
  • 目前的局限性包括超短核酸的检测不佳,依赖预放大,交叉污染风险,以及在上和非核酸目标的困难.

研究的目的:

  • 审查工程CRISPRRNA (crRNA) 设计CRISPR-Dx.最近的进展.
  • 要突出这些工程crRNAs如何解决基于CRISPR的诊断现有挑战.

主要方法:

  • 关于工程CRISPRRNA (crRNA) 修改和应用在基于CRISPR的诊断方面的文献综述.
  • 分析克服敏感性,特异性和目标范围的局限性的策略.

主要成果:

  • 工程crRNAs在检测超短核酸中表现出增强的能力.
  • 新设计减轻了预放大依赖,减少了交叉污染风险.
  • 在开发上检测和扩展目标检测到非核酸分子方面取得了进展.

结论:

  • 工程CRISPRRNA (crRNA) 代表了克服CRISPR-Dx局限性的重要一步.
  • 这些进步为基于CRISPR的更强大,更多功能,更易于使用的诊断工具铺平了道路.
  • 未来的研究应该专注于进一步优化crRNA设计并将其集成到护理点应用中.