Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Artificial repression looping control by regulated autoinhibition of transcription activator-like effector dimer proteins.

Nucleic acids research·2026

Same author

Defining the DNA Binding Specificity of GRHL2.

bioRxiv : the preprint server for biology·2026

Same author

Engineered transcription activator-like effector dimer proteins confer DNA loop-dependent gene repression comparable to Lac repressor.

Nucleic acids research·2024

Same author

High-Resolution Characterization of DNA/Protein Complexes in Living Bacteria.

Methods in molecular biology (Clifton, N.J.)·2024

Same author

Examining the Effects of Netropsin on the Curvature of DNA A-Tracts Using Electrophoresis.

Molecules (Basel, Switzerland)·2021

Same author

Designed architectural proteins that tune DNA looping in bacteria.

Nucleic acids research·2021

Search research articles

相关实验视频

Updated: Jun 20, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

用原子力显微镜测定DNA持久长度的方法

Justin P Peters^1,2, L James Maher Iii³

¹Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, MN, USA.

Methods in molecular biology (Clifton, N.J.)

|July 19, 2024

概括

此摘要是机器生成的。

原子力显微镜 (AFM) 揭示了DNA的机械性质如何随着基因修改而改变. 十种AFM方法组合提供可靠的DNA持久长度测量.

关键词:

原子力显微镜的原子力显微镜.DNA 机械学 DNA 机械持久的长度持久的长度提米丁类型的类似物类似虫的链条模型.

更多相关视频

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

相关实验视频

Last Updated: Jun 20, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

科学领域:

生物物理学的生物物理.
分子生物学分子生物学
材料科学材料科学材料科学

背景情况:

原子力显微镜 (AFM) 是可视化纳米尺度生物分子的关键技术.
了解DNA的机械性质,如持久长度,对于分子生物学至关重要.
静电相互作用和基层堆叠显著影响DNA聚合物力学.

研究的目的:

用AFM研究基因修改对DNA机械性质的影响.
为了确定静电学和基层堆叠强度如何影响DNA持久长度.
开发和验证基于AFM的多种方法,以准确测量DNA持久长度.

主要方法:

使用原子力显微镜 (AFM) 来成像双链DNA (dsDNA) 分子.
通过各种中性和充电基调的dSDNA进行研究.
采用十种互补的AFM成像方法来量化DNA持久长度.

主要成果:

证明了DNA机械性质对静电相互作用和基堆叠的依赖.
成功应用多种AFM方法来确定DNA持久长度.
通过结合的方法方法来提高统计可靠性和信心.

结论:

AFM是一种多功能工具,用于探测DNA的机械性质和基因修改的影响.
结合十种不同的AFM方法,提供了一种可靠的方法来测量DNA持久长度.
这种多方法策略与单一方法方法相比,提高了持久性长度确定的可靠性.