慢性乙肝病毒感染不同阶段的单细胞转录组揭示了潜在的功能性作用
Prakriti Sinha1, Vaishali Bhardwaj2, Abaranjitha Muniyasamy1
1National Institute of Immunology, New Delhi, India.
Viral immunology
|July 25, 2024
概括
乙型肝炎病毒 (HBV) 感染涉及不同的阶段. 单细胞基因表达分析揭示了每个阶段独特的分子特征,影响免疫细胞功能和病毒控制.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 文字转录学 (Transcriptomics) 是一个学科.
- 乙型肝炎病毒 (HBV) 感染
背景情况:
- 慢性乙型肝炎病毒 (HBV) 感染通过免疫耐受性 (IT),免疫清除 (IC) 和非活性 (IN) 阶段进展.
- 虽然适应性免疫是控制病毒的关键,但与生俱来的免疫细胞 (如单细胞) 的作用不太清楚.
- 了解单细胞参与对于破译HBV感染动态至关重要.
研究的目的:
- 为了研究慢性HBV感染的不同阶段的单细胞转录组.
- 为了确定每个感染阶段特有的独特差异表达基因 (DEGs).
- 阐明这些DEGs在HBV感染期间对单细胞行为的功能影响.
主要方法:
- 从IT,IC,IN阶段的患者和健康对照中分离CD14+外周血液单细胞.
- RNA测序以分析单细胞转录组.
- 对DEG和路径调制的生物信息分析.
- 定量逆转录PCR (qRT-PCR) 用于基因验证.
- 接收器运行特征 (ROC) 曲线分析用于诊断潜力.
主要成果:
- 在所有HBV感染阶段,TNFRSF12A的升级调节.
- IN阶段显示了六个基因的独特上调,包括PI3和SPINK1.
- 在IT阶段,E2F7被持续下调,而在IC阶段,IL23A和PI3被持续下调.
- 在IT和IC阶段,单细胞功能似乎被抑制,在IN阶段有一些代谢活动.
结论:
- 单细胞转录形状在HBV感染阶段之间有显著差异.
- 特定的基因在每个阶段都有独特的表达,为HBV进展提供了潜在的生物标志物.
- 单细胞的功能状态在不同阶段有所不同,在早期阶段抑制,在不活跃阶段部分恢复.
更多相关视频
10:25"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Published on: February 19, 2019
11.4K
10:37Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
2.9K
相关概念视频
Viruses with RNA Genomes
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Human Virome
The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
Hepatitis
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Viral Hepatitis I: Introduction
Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...
