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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
CRISPR01:59

CRISPR

50.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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相关实验视频

Updated: Jun 18, 2025

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
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Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

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使用CRISPECTOR2.0量化基因特异性的CRISPR编辑活动.

Guy Assa1, Nechama Kalter2, Michael Rosenberg2

  • 1Arazi School of Computer Science, Reichman University, Herzliya 4610101, Israel.

Nucleic acids research
|July 30, 2024
PubMed
概括

CRISPECTOR2.0量化了特定于每个DNA等位基因的基因组编辑活动. 该工具通过分析序列变异来解决非目标效应,以获得更安全,个性化的基因编辑应用.

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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相关实验视频

Last Updated: Jun 18, 2025

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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科学领域:

  • 遗传学和基因组学 遗传学和基因组学
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因组编辑CRISPR-Cas的非目标效应限制了它的安全应用.
  • 基因组序列变异会影响对基因基因组的目标和非目标编辑档案.
  • 目前还没有针对某个等位基因的基因组编辑量化工具.

研究的目的:

  • 介绍CRISPECTOR2.0,一个增强的软件用于基因组特异性基因组编辑量化.
  • 能够准确,无参考,并对目标和目标以外活动的等位基因意识测量.
  • 使用多种细胞类型验证CRISPECTOR2.0并确定影响等位基因特定编辑的因素.

主要方法:

  • 在CRISPECTOR2.0中,使用了新的单核酸变体 (SNV) 检测.
  • 采用基于统计的等位基调用算法进行精确的量化.
  • 分析来自人类细胞系,原始人类细胞和植物的数据.

主要成果:

  • CRISPECTOR2.0有效量化了具有多个等位基因的样本中的等位基因特定编辑活动.
  • 鉴定出SNVs会改变原空间体相邻的动图序列,导致异位基因特异性编辑.
  • 即使在远端SNV中也观察到差异性等位基编辑,这表明表观遗传的参与.

结论:

  • CRISPECTOR2.0提供了一种可靠的基因组编辑量化方法.
  • 了解基因组特异性编辑对于开发安全和个性化的基因组编辑策略至关重要.
  • 表观遗传因素可能在差异性等位基编辑结果中发挥作用.