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相关概念视频

The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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Replication in Prokaryotes01:32

Replication in Prokaryotes

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

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病毒复制器官:高度复杂和可编程的复制机器.

Hao Deng1, Hongwei Cao1, Yanjin Wang1

  • 1State Key Laboratory for Animal Disease Control and Prevention, National African Swine Fever Para-reference Laboratory, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

Frontiers in microbiology
|August 15, 2024
PubMed
概括

病毒感染产生复制器官 (ROs) 用于基因组复制. 本综述强调了RO中的关键病毒复制和针对未来战略的抗病毒药物的进展.

关键词:
DNA 滑动子 DNA 滑动抗病毒药物 抗病毒药物病毒DNA连接酶是病毒的DNA连接酶.病毒螺旋酶病毒螺旋酶病毒聚合酶病毒的聚合酶.病毒复制器官病毒复制器官

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

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Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
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科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 病毒感染重新安排宿主细胞结构,形成复制器官 (RO).
  • 病毒复制酶 (聚合酶,酶,酶) 对于RO内基因组复制是必不可少的.
  • 这些复制体是抗病毒药物开发的关键目标.

研究的目的:

  • 要总结在复制器官中发现的关键病毒复制体.
  • 突出针对这些关键病毒酶的抗病毒药物的进展.
  • 为未来的抗病毒药物开发战略提供见解.

主要方法:

  • 关于病毒复制机制的文献综述.
  • 对病毒复制酶及其功能研究的分析.
  • 对针对病毒复制物的当前和新兴抗病毒药物的调查.

主要成果:

  • 在RO中识别必要的病毒复制酶 (聚合酶,螺旋酶,酶).
  • 对针对这些病毒酶的药物开发进展的概述.
  • 讨论针对新型抗病毒疗法针对病毒复制物的潜力.

结论:

  • 在RO中病毒复制对病毒复制和有效的药物标至关重要.
  • 准病毒复制体为开发新的抗病毒策略提供了一个有希望的途径.
  • 对病毒复制物的持续研究和药物开发对于对抗病毒感染至关重要.