Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.9K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Longitudinal Monitoring of Metabolic Gradients in Microreactor Culture Platforms by Raman Spectroscopy.

Biosensors·2026
Same author

Fibroblast Activation Protein Alpha (FAP) Expression Is Associated with Disease Recurrence and Poor Response to Tyrosine Kinase Inhibitors in Advanced Clear Cell Renal Cell Carcinoma.

International journal of molecular sciences·2025
Same author

6-Shogaol Reduces Renal Macrophage Infiltration by Targeting the STING Pathway to Alleviate Cisplatin Induced Renal Injury.

Phytotherapy research : PTR·2025
Same author

Solving Mazes of Organelle-Targeted Therapies with DNA Nanomachines.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Quasi-photonic crystals-boosted highly efficient Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene photothermal materials for untethered actuators and therapeutics.

Journal of colloid and interface science·2025
Same author

Pressure Induced Molecular-Arrangement and Charge-Density Perturbance in Doped Polymer for Intelligent Motion and Vocal Recognitions.

Advanced materials (Deerfield Beach, Fla.)·2025

相关实验视频

Updated: May 5, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K

特定形状的金纳米粒子用于多重生物传感应用.

Shixi Zhang1,2, Yuhan Zhang1,2, Jiaye Jiang1,2

  • 1Sino-Swiss Institute of Advanced Technology (SSIAT), Shanghai University, Shanghai 201899, China.

ACS omega
|September 9, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的色度纳米生物传感器,用于同时检测流感和SARS-CoV-2病毒. 基于金纳米粒子的传感器提供快速,无设备的病原体识别,具有高灵敏度.

更多相关视频

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.2K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.6K

相关实验视频

Last Updated: May 5, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.2K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.6K

科学领域:

  • 纳米技术纳米技术
  • 生物感应是一种生物感应.
  • 病毒学 病毒学

背景情况:

  • 多重分析器检测对于诊断至关重要,但也面临着挑战.
  • 像PCR和ELISA这样的既定方法是有效的,但可能很复杂.
  • 对于多种病原体,需要快速,易于使用的诊断工具.

研究的目的:

  • 开发一种色度纳米生物传感器,用于同时检测A型流感病毒和SARS-CoV-2.
  • 为了多重检测,利用具有独特形态的功能化黄金纳米粒子.
  • 在没有专门设备的情况下创建可视化解释的诊断系统.

主要方法:

  • 功能化金纳米球 (GNSp) 含有流感A特定的寡核酸.
  • 功能化黄金纳米 (GNSh) 与SARS-CoV-2特定的寡核酸.
  • 利用目标诱导的DNA-DNA相互作用来控制纳米粒子聚合和颜色变化.

主要成果:

  • 纳米生物传感器表现出不同的色度反应:灰色表示SARS-CoV-2,蓝色表示流感A,紫色表示两者.
  • 实现了100nM的分析灵敏度,用于目标检测.
  • 没有证明与其他潜在病原体的交叉反应,确保特异性.

结论:

  • 开发的基于黄金纳米粒子的纳米生物传感器能够快速,同时和视觉检测流感A和SARS-CoV-2.
  • 这项技术为医疗诊断提供了一个有希望的替代方案,不需要专门的设备或人员.
  • 该系统解决了在单个样本中有效识别多重病原体的需求.