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相关概念视频

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
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由单细胞RNA-seqq揭示的牙组织衍生的MSC中的异质性.

C Behm1, O Miłek1, K Schwarz1

  • 1Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria.

Journal of dental research
|September 27, 2024
PubMed
概括

来自牙组织的间酶体 stromal 细胞 (MSC) 在组织和捐赠者之间显示出有限的变异性. 在单细胞水平上了解这些差异是有效的基于细胞的疗法的关键.

关键词:
基因表达分析 基因表达分析的 的 的介质细胞层细胞.牙周关节带 牙周关节带一个单细胞分析.翻译学 翻译学 翻译学 翻译学

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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科学领域:

  • 细胞生物学 细胞生物学
  • 再生医学是一种再生医学.
  • 基因组学就是基因组学.

背景情况:

  • 介酶体 stromal 细胞 (MSCs) 对治疗退行性和炎症性疾病充满希望.
  • 由于组织和供体变异的MSC异质性限制了治疗用途.
  • 单细胞转录组学对于理解MSC多样性至关重要.

研究的目的:

  • 调查从牙周带 (PDL) 和牙上产生的MSC的组织特异性和供体特异性转录组变异性.
  • 在牙组织中识别不同的MSC亚群.
  • 评估组织来源和个体捐赠者对MSC特征的影响.

主要方法:

  • 从PDL和5名健康个体的牙中分离和体外培养MSCs.
  • 3,844个转录组的单细胞mRNA测序.
  • 生物信息分析包括聚类,基因/通路丰富和蛋白质-蛋白质相互作用 (PPI) 网络分析.

主要成果:

  • 识别PDL和牙特异性和组织跨越的MSC亚群.
  • 组织特异性MSC亚群之间细胞过程的有限差异.
  • 在MSC转录组中观察到显著但受限的捐赠者对捐赠者的变异性.

结论:

  • 牙组织衍生的MSCs表现出特定于组织和供体的转录组变异,主要影响特定的细胞过程.
  • 在牙组织中存在不同的MSC亚群,突出显示细胞间差异.
  • 在移植之前,建议在单细胞水平上控制MSC特性,以治疗应用.