Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Challenges and progress in RNA velocity: Comparative analysis across multiple biological contexts.

PLoS computational biology·2026
Same author

Scalable single-cell total RNA sequencing unifies coding and noncoding transcriptomics.

Nature biotechnology·2026
Same author

Metagenomic surveillance reveals off-season circulation of respiratory viruses during the COVID-19 pandemic in Salvador, Brazil.

New microbes and new infections·2026
Same author

Implementation and outcomes of a rapid response genomic hospital epidemiology programme at an academic medical centre over 7 years.

The Lancet. Microbe·2026
Same author

PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.

Nature communications·2025
Same author

Comparative analysis of human and mouse ovaries across age.

Science (New York, N.Y.)·2025

相关实验视频

Updated: Jun 12, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

14.5K

为单个斑马鱼胚胎最大化单细胞解离协议.

Shruthi VijayKumar1, Michael Borja1, Norma Neff1

  • 1Chan Zuckerberg Biohub, San Francisco, USA.

MethodsX
|September 27, 2024
PubMed
概括

我们优化了斑马鱼胚胎的单细胞分离协议,使细胞异质性的详细研究成为可能,即使起始材料有限. 该方法支持对发育生物学和疾病机制的研究.

科学领域:

  • 发展生物学 发展生物学
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 单细胞测序对于理解细胞异质性和细胞状态至关重要.
  • 在制备高质量的单细胞悬浮剂方面存在挑战,特别是从斑马鱼胚胎等有限样本中.
  • 斑马鱼胚胎是发育生物学和疾病研究的重要模型.

研究的目的:

  • 提出一种优化的协议,用于从单个斑马鱼胚胎中分离单个细胞.
  • 为研究人员提供一个资源,以有限的起始材料工作.
  • 为了使用单个斑马鱼胚胎进行独特的研究.

主要方法:

  • 精制的单细胞隔离协议.
  • 使用斑马鱼胚胎作为模型生物.

主要成果:

  • 成功优化了从单个斑马鱼胚胎中分离单个细胞的协议.
  • 协议促进了独特的调查,如个体间的遗传差异和胚胎特异性血统追踪.
  • 为研究人员提供了宝贵的资源,研究人员的起始材料有限.

结论:

  • 优化的协议增强了斑马鱼胚胎在单细胞研究中的实用性.
关键词:
分离 分离 分离 分离.单个斑马鱼胚胎分离离单细胞机是一种单细胞机.斑马鱼是一种斑马鱼.

更多相关视频

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.8K
Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos
10:28

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

7.9K

相关实验视频

Last Updated: Jun 12, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

14.5K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.8K
Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos
10:28

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

7.9K
  • 能够探索细胞的出现,类型和状态.
  • 在发育背景下推进对细胞过程和疾病机制的理解.