Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:34

DNA Isolation

176.3K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
176.3K
DNA Isolation01:24

DNA Isolation

35.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
35.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Proteome and miRNAs Expression in Medication-Related Osteonecrosis of the Jaw.

International journal of molecular sciences·2026
Same author

Tubular Aggregate Myopathies: Genetic Heterogeneity and Diverse Clinical Features Converging on Calcium Dysregulation.

Cells·2026
Same author

Proteomic Profiling of Non-Muscle Invasive Bladder Cancer Reveals Stage-Specific Molecular Signatures and Prognostic Biomarkers.

Proteomes·2025
Same author

Molecular Insights into Central Core Disease: Proteomic Signatures and Potential Therapeutic Biomarkers in RYR1 I4895T Mice.

International journal of molecular sciences·2025
Same author

Investigating the Cytoprotective Mechanisms of the Tardigrade Damage Suppressor (Dsup) Protein in Human Cells Under Hypoxic Stress.

International journal of molecular sciences·2025
Same author

Estrogens and Antioxidants Prevent the Formation of Tubular Aggregates in Aging Male Mice.

International journal of molecular sciences·2025

相关实验视频

Updated: May 2, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.5K

适合肌肉组织蛋白质组分析的蛋白质提取方法

Lorenza Vantaggiato1, Claudia Landi1, Enxhi Shaba1

  • 1Functional Proteomics Lab., Department Life Sciences, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

Proteomes
|October 25, 2024
PubMed
概括
此摘要是机器生成的。

两种蛋白质提取方法用于肌肉组织蛋白质组学进行了比较. 这两种方法都被证明是有效和可重复的,产生了不同的蛋白质配置文件,用于全面分析.

关键词:
质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量肌肉组织 肌肉组织肌肉病变 (myopathies) 是一种神经病变.蛋白质变质化是蛋白质的变质化.两个维的电泳是二维的.

更多相关视频

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

12.6K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

356

相关实验视频

Last Updated: May 2, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.5K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

12.6K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

356

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 肌肉生物学 肌肉生物学
  • 生物化学 生物化学

背景情况:

  • 肌肉组织的动态性质和在力量生成和新陈代谢中的作用至关重要.
  • 了解肌肉病理生理学依赖于来自肌肉蛋白质学的分子见解.
  • 有效和可重复的蛋白质提取对于成功的蛋白质组分析至关重要.

研究的目的:

  • 为了评估两个不同的肌肉样本蛋白质提取协议的有效性.
  • 为了比较基于SDS的缓冲剂 (方法A) 和小鼠肌肉的UREA/CHAPS/DTE/TRIS溶液 (方法B).
  • 评估蛋白质提取对二维凝电泳 (2DE) 的适用性.

主要方法:

  • 用两种不同的缓冲系统提取小鼠肌肉蛋白:方法A (基于SDS) 和方法B (UREA/CHAPS/DTE/TRIS).
  • 通过对2DE凝的图像分析来评估蛋白质提取功效.
  • 使用统计和多变量分析来比较这些方法.

主要成果:

  • 方法A和方法B都产生了具有良好的分辨率和斑点重叠的2DE凝.
  • 与方法A相比,方法B的总斑点平均数量更高.
  • 图像分析揭示了两种协议之间明显的蛋白质丰度模式,表明蛋白质的提取不同,具有不同的特征和定位.

结论:

  • 基于SDS和UREA/CHAPS/DTE/TRIS的提取方法对于肌肉组织蛋白质基质分析都是有效和可重复的.
  • 这两种方法可使具有不同化学物理性质和细胞起源的蛋白质溶解.
  • 这些互补的方法可以并行用于全面的肌肉蛋白质组分析.