Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

1
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
1

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Design of bacterial DNT sensors based on computational models.

Nucleic acids research·2026
Same author

Bioluminescent Microbial Bioreporters: A Personal Perspective.

Biosensors·2025
Same author

Enhanced Detection of Estrogen-like Compounds by Genetically Engineered Yeast Sensor Strains.

Biosensors·2024
Same author

Performance upgrade of a microbial explosives' sensor strain by screening a high throughput saturation library of a transcriptional regulator.

Computational and structural biotechnology journal·2023
Same author

Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives' detection capabilities.

Engineering in life sciences·2022
Same author

Enhancing DNT Detection by a Bacterial Bioreporter: Directed Evolution of the Transcriptional Activator YhaJ.

Frontiers in bioengineering and biotechnology·2022

相关实验视频

Updated: Jun 9, 2025

Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.5K

一个微生物可卡因生物传递器

Anne-Kathrin Grimm1, Dor Rozanes2, Etai Shpigel2

  • 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93053 Regensburg, Germany.

Sensors (Basel, Switzerland)
|October 26, 2024
PubMed
概括

科学家们设计了一种全细胞细菌生物传递器,用于快速检测可卡因. 这种敏感的生物传感器为在各种环境中识别非法精神活性物质提供了有希望的工具.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.伪omonas putida可以使用.在Operon的Operon中,我们可以使用Operon.生物发光是一种生物发光.这是可卡因,可卡因.可卡因雌激酶可卡因雌激酶微生物生物转运器是微生物的生物转运器.

更多相关视频

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
13:16

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

10.3K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.7K

相关实验视频

Last Updated: Jun 9, 2025

Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.5K
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
13:16

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

10.3K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.7K

科学领域:

  • 微生物学 微生物学
  • 生物技术是生物技术.
  • 分析化学 分析化学

背景情况:

  • 新兴的非法精神活性化合物挑战了现有的药物检测方法.
  • 为每一种新的非法物质开发特定的测试是耗时和资源的.
  • 全细胞细菌生物传递器提供了一个多功能平台,用于检测各种危险化学品和化学品家族.

研究的目的:

  • 开发一种微生物生物传递器,用于敏感和特定检测广泛使用的非法兴奋剂可卡因.
  • 用可卡因反应的生物发光系统来设计大肠杆菌,以便快速识别.
  • 优化生物报告器的性能,并评估其在现实世界样本矩阵中的适用性.

主要方法:

  • 在可卡因敏感促进体的控制下,用编码*luxCDABEG*生物发光基因的等离子体转化大肠杆菌.
  • 使用加速位点导向进化,优化传感元件 (*PBen*基因促进体) 的优化.
  • 在缓冲溶液和尿样中验证生物报告者的敏感性和特异性.
  • 在纸条上使用固定生物记者细胞检测可卡因的演示.

主要成果:

  • 工程生物记者表现出敏感的可卡因检测,缓冲剂的值为17±8μg/L,尿液的值为130±50μg/L.
  • *PBen*促进器的站点导向进化提高了传感器的性能.
  • 生物报告器系统证明有效地检测可卡因在水溶液中,使用纸条上的固定细胞.

结论:

  • 一个用于可卡因检测的新型微生物生物传递系统已经成功开发出来.
  • 工程生物传感器具有高灵敏度和特异性,为药物监测提供了有价值的工具.
  • 基于纸条检测的应用突出了快速,现场非法物质识别的潜力.