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相关概念视频

Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Prokaryotic Transcriptional Activators and Repressors01:58

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
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Updated: Jun 9, 2025

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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对于Streptomyces的光可诱导基因表达系统.

Ryuta Noya1, Kyohei Murakoshi1, Madoka Fukuda1

  • 1Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-0880, Japan.

Scientific reports
|October 29, 2024
PubMed
概括

研究人员使用Litr和Lits调节器在Streptomyces细菌中开发了一种可诱导光系统. 这种系统使酶和二次代谢物的过度生产成为可能,为微生物生物技术提供了新的可能性.

关键词:
这种病名为Streptomyces.具有光诱导性的在LitRR LitRR中使用.视觉遗传学 视觉遗传学再组合蛋白质是一种重组蛋白质.

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科学领域:

  • 微生物学 微生物学
  • 合成生物学 合成生物学
  • 生物技术是生物技术.

背景情况:

  • LitR/CarH家族是光感受调节剂,控制细菌中可感应光的胡卜素的产生.
  • 非光合作用细菌利用这些系统进行依赖光的代谢过程.

研究的目的:

  • 使用LitR和LitsS.在Streptomyces griseus中建立一个蓝绿色的可诱导光的过度表达系统.
  • 为了证明系统对各种分子的过度生产的能力.

主要方法:

  • 构建了一个多复制的等离子体 (pLit19),其中包含用于光感应表达的关键遗传元素.
  • 引入的pLit19进入了Streptomyces griseus和Streptomyces sp.中的一个. 这是NBRC 13304.4.
  • 通过插入一个sti片段并使用航天飞机类型的等离子体来测试光敏度.

主要成果:

  • 变态菌株Streptomyces显示出细胞内和细胞外酶的依赖光的过度生产.
  • 观察到光可诱导的二次代谢物如黑色素和actinorhodin的产生.
  • 实现了增强的光敏感性,使得在弱光下表达.

结论:

  • 成功建立了对Streptomyces的光遗传控制的过度生产系统.
  • 开发的系统允许精确控制使用光的基因表达.
  • 该系统在微生物生物技术和二次代谢物生产中具有潜在的应用.