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Updated: Jun 7, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

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GENCODE:通过捕获长读RNA测序来大规模扩展lncRNA目录

Gazaldeep Kaur1, Tamara Perteghella1,2, Sílvia Carbonell-Sala1

  • 1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Catalonia, Spain.

bioRxiv : the preprint server for biology
|November 18, 2024
PubMed
概括

GENCODE联盟在人类和小鼠基因组中显著扩展了长非编码RNA (lncRNA) 标注. 这一全面的更新增强了对基因功能和疾病关联的理解.

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RNA-seq03:21

RNA-seq

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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