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相关概念视频

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
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薄层子宫样本评估:传统光显微镜与数字整片成像相比.

Jessica Viti1, Chiara Di Stefano1, Serena Giunti1

  • 1Regional Laboratory of Cancer Prevention, Institute for Cancer Research, Prevention and Clinical Network (ISPRO), Florence, Italy.

Cancer cytopathology
|November 21, 2024
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概括

整片成像 (WSI) 显示了与常规光显微镜 (CLM) 评估宫细胞学的实质一致性,除了ASC-US和ASC-H等边界类别. WSI技术的进一步进步是必要的,以实现常规诊断使用.

关键词:
细胞病理学 细胞病理学数字病理学数字病理学光显微镜光学显微镜整个幻灯片成像成像技术

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科学领域:

  • 病理学 病理学 病理学
  • 细胞病理学 细胞病理学
  • 数字病理学数字病理学

背景情况:

  • 全幻灯片成像 (WSI) 在病理学中越来越多地用于诊断和教育.
  • 美国病理学家学院 (CAP) 在2021年更新的指南支持WSI系统验证.
  • 现有的文献主要集中在组织病理学上,在细胞学中对WSI的关注较少.

研究的目的:

  • 将常规光显微镜 (CLM) 与WSI进行比较,用于评估薄层子宫样本.
  • 在细胞病理学中根据CAP指南评估WSI的表现.
  • 为了确定各种细胞病理学类别的一致率,使用WSI与CLM.

主要方法:

  • 分析了来自25-64岁妇女的64个薄层子宫样本.
  • 五位细胞学家进行了CLM分析.
  • 同样的幻灯片后来在多个放大时使用WSI进行了评估.

主要成果:

  • 对于NILM,CLM和WSI之间的实质性到近乎完美的一致性 (κ ≥0.77) (内皮层病变或恶性病变负).
  • 在细胞学家中观察到LSIL,HSIL和AGC的可变一致率.
  • 边界类别ASC-US和ASC-H的下一个协议 (κ ≤ 0.39)

结论:

  • 在大多数宫细胞学类别中,WSI与CLM有实质性或近乎完美的一致性.
  • 边界类别 (ASC-US,ASC-H) 的WSI表现不那么一致.
  • 需要进一步开发WSI系统,以便将其整合到常规细胞病理诊断中.