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相关概念视频

RNA Splicing01:32

RNA Splicing

56.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
What is Gene Expression?01:36

What is Gene Expression?

8.4K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.4K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

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相关实验视频

Updated: Jun 6, 2025

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

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在一个大规模的多发性硬化症研究中,基因表达和替代拼接分析.

Müge Sak1, Julia H Chariker1, Juw Won Park2

  • 1Kentucky IDeA Networks of Biomedical Research Excellence Bioinformatics Core, Department of Neuroscience Training, University of Louisville, Louisville, KY 40292, USA.

International journal of molecular sciences
|November 27, 2024
PubMed
概括
此摘要是机器生成的。

这项研究揭示了多发性硬化症 (MS) 白质中的新型基因表达和拼接变化,确定了这种自身免疫性神经退行性疾病的潜在新药标.

关键词:
在RNA-seqqq.替代性拼接是一种替代性的拼接.不同的表达方式,不同的表达方式.多发性硬化症多发性硬化症

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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相关实验视频

Last Updated: Jun 6, 2025

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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科学领域:

  • 神经免疫学 神经免疫学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 多发性硬化症 (MS) 是一种重要的自身免疫性神经退行性疾病,影响全球数百万人.
  • 尽管进行了广泛的研究,但驱动多发性硬化发展和进展的确切机制仍然不完全理解.
  • 了解MS中的分子变化对于确定有效的治疗策略至关重要.

研究的目的:

  • 研究MS患者和健康对照患者死后白质中的基因表达模式和替代拼接事件.
  • 确定与MS病变发生相关的分子途径和潜在的病理异型.
  • 发现用于多发性硬化症治疗的新药标.

主要方法:

  • 从MS患者和对照捐赠者的公开可用的RNA测序数据集 (GSE138614) 的分析.
  • 基因表达水平与组织炎症标志物的相关性.
  • 识别RNA结合基因,差异表达的RNA结合蛋白和单核酸多态 (SNP).

主要成果:

  • 与炎症正相关的基因在免疫和受体通路中得到丰富;负相关的基因在神经系统发育和新陈代谢中得到丰富.
  • 在看起来正常的白质 (NAWM) 和活跃的MS病变之间观察到不同的基因表达特征.
  • 在关键基因 (如MARCHF1和UGT8) 中发现了外显子跳转和自发SNP,这些基因与自身免疫和神经退行有关.

结论:

  • 该研究确定了导致MS病理的独特基因,途径和拼接事件.
  • 在MS白质中,特定的基因和拼接变化为新型治疗干预提供了潜在的目标.
  • 对这些发现的进一步调查可能会导致改善多发性硬化症的治疗方法.