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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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相关实验视频

Updated: Jun 6, 2025

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

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在RNA药物开发的最新进展和前景.

Hidenori Tani1

  • 1Department of Health Pharmacy, Yokohama University of Pharmacy, 601 Matano, Totsuka, Yokohama 245-0066, Japan.

International journal of molecular sciences
|November 27, 2024
PubMed
概括
此摘要是机器生成的。

RNA疗法,包括反感性寡核酸 (ASO) 和信使RNA (mRNA),已经显著发展,为疾病提供了新的治疗方法. 交付和人工智能的进步解决了挑战,为个性化医疗铺平了道路.

关键词:
在CRISPR-Cas9指导RNA中.对于RNA疗法来说,它是非常重要的.反感的寡核化物.它们是Aptamers.传递 RNA 的信使 RNA.微RNAs 是一个微型RNA.小的干扰RNAs可以干扰.

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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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科学领域:

  • 生物技术和分子医学 生物技术和分子医学
  • 遗传学和基因组学 遗传学和基因组学
  • 药物发现和开发 药物发现和开发

背景情况:

  • 自20世纪70年代以来,RNA疗法一直在发展,彻底改变了对难以治疗疾病的医学.
  • 关键的方法包括反意义寡核酸 (ASO),小干扰RNA (siRNA),微RNA (miRNA) 和信使RNA (mRNA).
  • 随着COVID-19的流行,人们对mRNA疫苗技术的兴趣加快了,并得到了验证.

研究的目的:

  • 提供关于RNA疗法的演变,当前状态和未来前景的全面概述.
  • 突出该领域的关键里程碑和新兴趋势.
  • 讨论基于RNA的药物开发的挑战和进展.

主要方法:

  • 历史发展和科学文献的回顾.
  • 分析当前的RNA治疗方式及其机制.
  • 检查最近的技术进步和临床成功.

主要成果:

  • 在开发基于RNA的各种遗传疾病和癌症的治疗方法方面取得了重大进展.
  • 经批准的脊柱肌肉缩和遗传性跨甲基素介导的粉症疗法证明了临床疗效.
  • 目前正在进行的研究重点是克服稳定性,输送,非目标效应和免疫性等挑战.

结论:

  • RNA疗法是一个快速发展的领域,具有个性化医学的变革潜力.
  • 技术创新,包括化学修饰,先进的交付系统和人工智能,对于未来的成功至关重要.
  • 持续的研发有望为广泛的疾病提供新的治疗方法.