Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Proteasome02:18

The Proteasome

8.5K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
8.5K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

3.5K
3.5K
Amino Acid Catabolism01:18

Amino Acid Catabolism

1
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
1
Regulated Protein Degradation02:58

Regulated Protein Degradation

2.5K
2.5K
The Proteasome Structure01:17

The Proteasome Structure

697
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
697
Protein Digestion01:02

Protein Digestion

102.6K
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
102.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Unraveling Metabolic Syndrome: Dr Pories Does It Again.

Journal of the American College of Surgeons·2026
Same author

Safety considerations for cereblon-recruiting targeted protein degraders.

Nature reviews. Drug discovery·2026
Same author

Optimizing Safety and Outcomes in Metabolic/Bariatric Surgery: A Conceptual Framework for Staged Minimally Invasive Treatment.

Obesity surgery·2026
Same author

Toxicologic Pathology Forum*: Summary of the 2024 Society of Toxicologic Pathology Town Hall and 2025 STP Member Survey on Determining and Communicating Adversity.

Toxicologic pathology·2025
Same author

Early experience of simultaneous sleeve gastrectomy in living donor liver transplant recipients with obesity - a pilot study.

Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery·2025
Same author

STP Town Hall Discussion on the Use of Virtual Control Groups in Nonclinical Toxicity Studies.

Toxicologic pathology·2025

相关实验视频

Updated: Jun 5, 2025

Tuning Degradation to Achieve Specific and Efficient Protein Depletion
05:11

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

6.2K

第五节:蛋白质降解剂

Kiran Palyada1, Renee Hukkanen2, Stephanie Leuenroth-Quinn3

  • 1Pfizer Inc., La Jolla, California, USA.

Toxicologic pathology
|December 11, 2024
PubMed
概括
此摘要是机器生成的。

向蛋白质降解 (TPD) 提供了一种新方法,通过利用细胞的自然降解途径,为以前无法降解的蛋白质开发药物. 这次会议探讨了TPD的挑战,毒性和发展考虑.

关键词:
E3 结合酶的使用这就是PROTAC.这是VHLVHL.这就是为什么Cereblon.不同生物功能分子的分子.分子粘剂分子粘剂蛋白质降解剂 蛋白质降解剂

更多相关视频

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.7K
Assaying Proteasomal Degradation in a Cell-free System in Plants
07:43

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

14.4K

相关实验视频

Last Updated: Jun 5, 2025

Tuning Degradation to Achieve Specific and Efficient Protein Depletion
05:11

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

6.2K
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.7K
Assaying Proteasomal Degradation in a Cell-free System in Plants
07:43

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

14.4K

科学领域:

  • 药物开发 药物开发
  • 生物化学 生物化学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 这是一个很棒的节目,这是一个很棒的节目.
  • 没有药物可用的无毒药.
  • 蛋白质组在药物发现中提出了重大挑战.
  • 向蛋白质降解 (TPD) 已成为解决这一挑战的有希望的治疗方式.
  • TPD利用细胞内源性无素-蛋白酶体系统进行向的蛋白质清除.

研究的目的:

  • 探索利用蛋白质降解剂作为新型治疗剂的挑战和前景.
  • 讨论与TPD相关的目标和目标外毒性评估.
  • 为开发和评估降解分子的安全性提出独特的考虑.

主要方法:

  • 审查当前的TPD策略及其在药物开发中的应用.
  • 通过IQ财团工作组调查分析目标和目标之外的毒性.
  • 降解分子独特的吸收,分布,新陈代谢和分泌 (ADME) 特性.
  • 讨论如何使用转基因模型来评估血毒性.
  • 一个案例研究说明了剂量限制性血小板缺血的降低风险.
  • 包括对TPD相关毒性的监管视角.

主要成果:

  • TPD的模式正在从学术界转移到工业界,提供新的治疗途径.
  • 降解分子的特定ADME特性影响药物开发和非临床安全.
  • 血毒性,特别是血小板缺血,是一个关键的安全问题,需要仔细评估.
  • 转基因模型和案例研究为管理TPD相关毒性提供了宝贵的见解.

结论:

  • 蛋白质降解剂在向以前无法降解的蛋白质方面取得了重大进展.
  • 解决ADME独特的特性和潜在的毒性,如血液毒性,对于成功开发TPD至关重要.
  • 包括监管考虑在内的多学科方法对于推进TPD疗法至关重要.