编码由猿类淋巴细胞病毒编码的微RNA的序列分析.
Yan Chen1, Devin N Fachko1, Helen L Wu2
1Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA.
Viruses
|January 8, 2025
概括
这项研究全面注释了来自非人类灵长类淋巴结核病毒 (LCV) 的超过95种病毒微RNA. 在循环中检测到类似的LCV微RNA,显示了与LCV相关的移植后淋巴增殖性疾病的潜在生物标志物.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 淋巴结核病毒 (LCV) 是一种导致灵长类动物终身感染的马疹病毒.
- 免疫功能低下的主体的LCV感染与B细胞疾病和淋巴瘤有关.
- 猿类LCV感染了各种非人类灵长类动物 (NHP) 种类,包括和.
研究的目的:
- 在四种NHP物种中,通过LCV编码的病毒微RNA (miRNA) 进行全面的注释.
- 为了研究猿类LCVmiRNAs作为LCV相关疾病的生物标志物的潜力.
主要方法:
- 来自毛里求斯Cynomolgus,,尾和牛的LCV基因组的序列比较分析.
- 使用生物信息学方法识别和注释病毒miRNAs.
- 使用分子分析检测循环中的cyLCVmiRNAs.
主要成果:
- 来自四个NHP LCV的95多个病毒miRNA的定义序列,代表了一个全面的注释.
- 在体内循环的体液中证明了cyLCVmiRNAs的存在.
- 鉴定了cyLCV miRNAs在诊断LCV相关的移植后淋巴增殖性疾病 (PTLD) 中的潜在生物标志物实用性.
结论:
- 这项研究提供了迄今为止NHP LCV miRNAs最广泛的目录.
- 已识别的病毒miRNAs作为开发诊断试剂的宝贵资源.
- 循环的cyLCVmiRNAs显示出作为PTLD的非侵入性生物标志物具有前途.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:30siRNA - Small Interfering RNAs
16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K
02:33Retroviruses
12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
00:36Viral Mutations
32.1K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.1K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K