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相关概念视频

RNA Splicing01:32

RNA Splicing

56.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
pre-mRNA Processing02:01

pre-mRNA Processing

52.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.6K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.2K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.5K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.5K

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相关实验视频

Updated: Jun 2, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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RNA拼接:一个分裂的共识揭示了两个主要的5'拼接位点类.

Matthew T Parker1, Sebastian M Fica2, Gordon G Simpson3

  • 1Max Planck Institute for Plant Breeding Research , Cologne, Germany.

Open biology
|January 14, 2025
PubMed
概括
此摘要是机器生成的。

人类5'拼接部位有两个不同的类别,基于RNA相互作用,影响基因拼接. 了解这些类别有助于理解基因组结构和治疗与拼接相关的疾病.

关键词:
在METTL16中,METTL16是METTL16中的一个.这是ReNU综合征.在SNRNP27K.这是一个T-Loop.m6A 一个很好的.拼接 拼接 拼接 拼接

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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相关实验视频

Last Updated: Jun 2, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • 标准的人类5'拼接部位序列隐藏了两个不同的类别.
  • 这些类表现出不同的基配对潜力与U5 snRNA循环1和U6 snRNA ACAGA盒.

研究的目的:

  • 区分和描述两个主要的人类5'拼接位点类.
  • 探索这些课程对拼接承诺和疾病的影响.

主要方法:

  • 对人类基因组序列的生物信息分析.
  • 对基因型特异性拼接变异的研究.
  • 检查拼接部位转移动态到U6 snRNA的检查.

主要成果:

  • 确定了两个主要的5'拼接部位类,可在基因组序列中分离.
  • 这些类与特定的基因型和增加的拼接复杂性有关.
  • 结合部位对U6 snRNA使用的承诺主要在此转移期间确定.

结论:

  • 人类5'拼接部位共识包括两个不同的功能组.
  • 这种分类为真核生物基因组组织和拼接提供了洞察力.
  • 这些发现可以为影响拼接的遗传疾病的治疗策略提供信息.