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Updated: May 31, 2025

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革命性的组织清除和三维成像:透明嵌入溶剂系统,用于统一的高分辨率成像

Yating Yi1, Hu Zhao2

  • 1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

BME frontiers
|January 24, 2025
PubMed
概括

像TESOS这样的透明嵌入系统可以为3D成像提供一致的亚微米分辨率. 这种技术对连接组映射和先进的多重组成像应用具有前景.

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Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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