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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K

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相关实验视频

Updated: May 27, 2025

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

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用胺单酸盐光亲和探针进行化学转录形状分析.

Stefan Crielaard1, Casper F M Peters1, Alexandar Slivkov1

  • 1Institute of Molecules and Materials, Radboud University Heyendaalseweg 135 Nijmegen 6525 AJ The Netherlands Willem.Velema@ru.nl.

Chemical science
|February 19, 2025
PubMed
概括

研究人员使用了一种新的化学转录组学方法,在大肠杆菌中发现了新的RNA-小分子相互作用. 他们发现胺单酸盐 (TMP) 与 рибо开关相互作用,这表明TMP的潜在生物作用.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 基因组学就是基因组学.

背景情况:

  • 带状切换器是RNA分子,通过结合小分子来调节基因表达.
  • 了解RNA-小分子相互作用对于破译细胞调节网络至关重要.

研究的目的:

  • 为了分析大肠杆菌转录组与胺单酸盐 (TMP) 的相互作用.
  • 通过化学转录基因分析识别新的RNA-小分子相互作用.

主要方法:

  • 基于TMP的光亲和探针的设计和合成.
  • 使用下一代RNA测序进行化学转录分析.
  • 使用RT-qPCR,竞争测试和SHAPE实验验证相互作用的验证.

主要成果:

  • 确定了TMP探针和细菌转录之间的潜在相互作用.
  • 验证了TMP探针与弗拉文单核酸 (FMN) 核糖开关之间的显著相互作用.
  • 局部化了光交叉连接核酸与 рибо开关胺体的核酸,并证实了TMP的中度结合.

结论:

  • 化学转录形状分析是发现新的RNA-小分子相互作用的可行方法.

更多相关视频

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

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相关实验视频

Last Updated: May 27, 2025

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

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  • 胺单酸盐 (TMP) 选择性地与FMN рибо开关结合,可能影响基因表达.
  • TMP可能具有与其天然连接体,FMN.不同的生物学作用.