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Updated: Jul 29, 2026

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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优化固相蛋白质合成使用CPG-2000和一个切割性SNAC标签链接器

Jianyi Han1,2,3,4, Bobo Dang1,2,3,4

  • 1College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.

Organic letters
|February 24, 2025
PubMed
概括

使用受控孔玻璃和可切割链接器的优化固相化学联结 (SPCL) 成功合成了一种131氨基酸蛋白质. 这种改进的方法实现了25%的 de novo 蛋白质合成产量.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...