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相关概念视频

Law of Independent Assortment02:03

Law of Independent Assortment

While Mendel’s Law of Segregation states that the two alleles for one gene are separated into different gametes, a different question of how different genes are inherited remains. For example, is the gene for tall plants inherited with the gene for green peas? Mendel asked this question by experimenting with a dihybrid cross; a cross in which both parents are homozygous for two distinct traits resulting in an F1 generation that are heterozygous for both traits.
Extraction: Partition and Distribution Coefficients01:14

Extraction: Partition and Distribution Coefficients

The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
For extracting a solute from an aqueous phase into an organic...
Hypothesis Test for Test of Independence01:16

Hypothesis Test for Test of Independence

The test of independence is a chi-square-based test used to determine whether two variables or factors are independent or dependent. This hypothesis test is used to examine the independence of the variables. One can construct two qualitative survey questions or experiments based on the variables in a contingency table. The goal is to see if the two variables are unrelated (independent) or related (dependent). The null and alternative hypotheses for this test are:
H0: The two variables (factors)...
Multiple Comparison Tests01:13

Multiple Comparison Tests

Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
Thevinin's Theorem01:15

Thevinin's Theorem

Thévenin's theorem plays a pivotal role in electrical circuit analysis, offering a solution to the challenges posed by variable loads within a circuit. In practical applications, it is common to encounter circuits where certain elements remain fixed while others fluctuate, often referred to as the "load." A typical household electrical outlet serves as a prime example of a variable load, as it can be connected to a variety of appliances, each with its own unique electrical characteristics.

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相关实验视频

Updated: Jul 10, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

12.8K

使用定量不可逆转连接试验对共价片段进行选.

Charles P Brown1, Alan Armstrong2, David J Mann3

  • 1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub; Department of Life Sciences, Imperial College London, South Kensington Campus.

Journal of visualized experiments : JoVE
|March 17, 2025
PubMed
概括

定量不可逆转连接 (qIT) 是一种用于药物发现的新方法,用于寻找共价联体. 它准确地测量化合物反应性,优先选择性共价抑制剂用于治疗开发.

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Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
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Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

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相关实验视频

Last Updated: Jul 10, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

12.8K
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

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科学领域:

  • 生物化学 生物化学
  • 化学生物学 化学生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 共价化合物通过与标蛋白形成键,作为化学探针和治疗药物提供优势.
  • 电-第一个连接体的发现很受欢迎,但由于与氨酸的不同化合物反应性而复杂.
  • 定量不可逆转连接 (qIT) 通过规范化内在化合物反应性的数据来解决这一问题.

研究的目的:

  • 为了证明 qIT 的应用,以生成有关联体-蛋白相互作用的定量数据.
  • 为进一步开发药物发现优先考虑击中配体.
  • 验证qIT作为确定选择性共价抑制剂的可靠方法.

主要方法:

  • 使用基于光的方法 (qIT) 进行命中识别和开发.
  • 测量单个化合物与目标蛋白质的反应速度.
  • 将化合物与目标蛋白的反应性与谷氨的反应性进行比较,作为自发反应的对照.

主要成果:

  • 通过使用qIT.成功生成了一个目标蛋白质的定量和强大的数据集.
  • 根据它们与目标蛋白质的优先反应能力,启用了命中配体的优先排序.
  • 证明了qIT在识别选择性共价片段方面的有效性,基于之前的成功.

结论:

  • qIT是一个强大的工具,用于识别和开发选择性共价配体.
  • 该方法对内在化合物反应性进行正常化,从而导致更可靠的击中优先级.
  • qIT促进了针对治疗应用的向共价抑制剂的发现.