在产后发育过程中,皮层第6层输入到第一阶的乳头核和乳头网状核的进展.
Guela Sokhadze1,2, Gubbi Govindaiah1, Peter Campbell1,3
1Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, Kentucky, USA.
The European journal of neuroscience
|March 18, 2025
概括
第六层 (L6) 皮质体膜 (CT) 投射到质体,以协调的顺序发展. 体网状核 (TRN) 中的连接在第一阶体核中的连接之前成熟,例如腹腔基核 (VB) 和背侧生殖细胞核 (dLGN).
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 系统神经科学 系统神经科学
背景情况:
- 新皮质中的第6层 (L6) 神经元形成了主要的激发性投射到 thalamus.
- 皮质体内皮层 (CT) 投射与体内皮层网状核 (TRN) 相互作用,影响体内皮层信号传递.
- 在TRN和thalamic核中L6CT连接的发展时间表在很大程度上是未知的.
研究的目的:
- 调查L6 CT轴突内化和TRN和第一阶梯形状细胞核 (VB,DLGN) 中功能性突触形成的发育时间.
- 在这些发展中的连接中描述突触功能的成熟.
主要方法:
- 使用L6特定的Nsr1-Cre鼠标线与Cre依赖的记者 (Ai9,Ai32) 交叉,以可视化和电生理学评估CT投影.
- 在体内对 tdTomato 标记的 CT 轴突进行成像,并在体外使用光遗传学进行急性切片电生理学以记录突触反应.
主要成果:
- 在出生后 (P) 2-3日,L6 CT轴突激活了TRN和腹侧VB,并在P7.7日扩展到所有VB.
- 背侧生殖核 (dLGN) 的内化从P7到P14之间从腹部向背部进展.
- 在TRN (P7),VB (P7-10) 和dLGN (P10-14) 中,功能激发后突触反应顺序出现,最初较弱,随着年龄的增长而成熟,显示突触促进.
结论:
- thalamus 的 L6 皮层内化是一个高度协调的发育过程.
- 在TRN内部的连接比第一阶质核 (VB,DLGN) 中的连接成熟得更早.
- 这种发育序列表明,在 thalamus 中建立调制反回路的特定顺序.
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