Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

12.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
12.9K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Plant Vacuolar and Human Endolysosomal Two-Pore Channels: Similarities and Differences.

Cells·2026
Same author

Resolving fluorescently labeled species using highly multiplexed spectral FLIM.

Scientific reports·2026
Same author

Expanded applications of bioluminescence microscopy with phasor analysis.

Cell reports methods·2026
Same author

Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1<sup>R132H</sup> Glioma Cell Lines.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Bioluminescent Probes for Multiplexed RNA Imaging.

Journal of the American Chemical Society·2026
Same author

Wavefront estimation through structured detection in laser scanning microscopy.

Biomedical optics express·2026

相关实验视频

Updated: May 21, 2025

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.5K

通过基于相机的SPLIT-ISM在图像扫描显微镜中利用探测器距离信息.

Elisabetta Di Franco1,2,3, Giulia Tedeschi3, Lorenzo Scipioni3

  • 1Department of Physics and Astronomy "Ettore Majorana", University of Catania, Catania, Italy.

Biomedical optics express
|March 20, 2025
PubMed
概括

图像扫描显微镜 (ISM) 的分辨率通过将其与通过终身调整 (SPLIT) 分离光子相结合来增强. 这种新的SPLIT-ISM技术提高了现有硬件上的生物成像分辨率和光学分割.

更多相关视频

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.3K

相关实验视频

Last Updated: May 21, 2025

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.5K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.3K

科学领域:

  • 生物光子学 生物光子学
  • 显微镜的使用方法
  • 超高分辨率成像成像技术

背景情况:

  • 混焦显微镜使用针孔来提高分辨率,但受到信号损失的限制.
  • 图像扫描显微镜 (ISM) 通过探测器阵列和像素重新分配来克服这一问题.
  • 用寿命调 (SPLIT) 分离光子是一种使用光寿命分析的超分辨率技术.

研究的目的:

  • 通过将图像扫描显微镜 (ISM) 与光子分离通过终身调 (SPLIT) 技术集成来提高图像扫描显微镜 (ISM) 的分辨率.
  • 调查SPLIT-ISM对横向分辨率和光学分割的影响.
  • 在商用ISM系统上验证SPLIT-ISM性能.

主要方法:

  • 通过分析ISM内的阵列探测器距离信息,开发了SPLIT-ISM.
  • 将SPLIT-ISM应用于使用热那亚仪器PRISM和Zeiss Airyscan ISM系统获得的生物图像.
  • 使用QuICS算法评估SPLIT-ISM性能,以量化分辨率和SNR.

主要成果:

  • 与标准像素重新分配的ISM相比,SPLIT-ISM实现了额外1.3倍的侧向分辨率增加.
  • 该技术表明,光学切割能力的同时增加.
  • 定量分析证实了两个测试的ISM系统的空间分辨率和SNR的改进.

结论:

  • SPLIT-ISM有效地提高了现有的ISM系统的分辨率和光学分割.
  • 将SPLIT与ISM集成为生物成像能力提供了重大进展.
  • SPLIT-ISM为高分辨率的生物成像应用提供了宝贵的工具.