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相关概念视频

Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Affinity and Avidity01:41

Affinity and Avidity

Overview
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

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相关实验视频

Updated: Jun 23, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

探索阿普坦酶结合热力学,亲和力和特异性之间的关系.

Obtin Alkhamis1, Caleb Byrd1, Juan Canoura1

  • 1Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695, United States.

Nucleic acids research
|March 29, 2025
PubMed
概括
此摘要是机器生成的。

阿普塔默-连接体相互作用主要由力驱动,具有与强结合力和性惩罚相关的高特异性. 这种热力学洞察力有助于aptamer工程改善结合性质.

更多相关视频

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

相关实验视频

Last Updated: Jun 23, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 胺是寡核酸生物受体,在诊断,生物成像和治疗方面越来越多地使用.
  • 了解体 - 体相互作用对于设计更好的体至关重要.
  • 很少有研究全面地将阿普坦酶结合特性与热力学联系起来.

研究的目的:

  • 系统地分析DNA体的热力学结合数据.
  • 探索阿普坦酶结合热力学与特异性之间的关系.
  • 为了识别aptamer-ligand相互作用中的模式.

主要方法:

  • 编译了来自317个小分子结合DNA吸收体的异热定位热量计 (ITC) 数据.
  • 分析了大约6000个阿普坦 - 连接体对的特异性概况.
  • 对编制的热力学和特异性数据集进行了系统分析.

主要成果:

  • 对于大多数体酶来说,体结合主要是由度驱动的.
  • 具有更高结合度和更大的罚的阿普塔默具有更高的特异性.
  • 鉴定了在阿普坦 - 连接体结合热力学中的明显模式和趋势.

结论:

  • 这项研究为aptamer-ligand识别提供了宝贵的热力学见解.
  • 这些发现可以指导序列工程,以提高aptamer性能.
  • 进一步的数据集扩展和分析将加深对胺酶结合机制的理解.