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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jan 15, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

无反应剂的实时ATP监测使用新DNA Aptamers.

Adara Bacon¹, Obtin Alkhamis¹, Caleb Byrd¹

¹Department of Chemistry, North Carolina State University, 2620 Yarbrough Dr., Raleigh, NC, 27695, USA.

Small (Weinheim an der Bergstrasse, Germany)

|October 16, 2025

概括

研究人员开发了一种新的DNA吸附体,ATP18-13,用于高度特定和敏感的实时检测腺三酸盐 (ATP). 这一突破改善了现有的生物传感器,使生物样本能够精确监测.

关键词:

这就是ATPATPATP ATP.这就是SELEXEX.它们是Aptamers.监控监控监控监控监控监控传感器传感器传感器

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Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Published on: March 11, 2021

相关实验视频

Last Updated: Jan 15, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Published on: March 11, 2021

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

腺三酸盐 (ATP) 是生物过程中的关键分子.
现有的ATP传感器在特异性,试剂要求和复杂性方面存在局限性.
目前用于ATP检测的阿普坦体在实际应用中表现出不足的亲和力和特异性.

研究的目的:

发现具有高亲和力和特异性的新型DNA体,对腺三酸盐 (ATP).
为实时ATP检测开发一种改进的以aptamer为基础的生物传感器.
为了证明传感器在监测涉及ATP的生物过程中的实用性.

主要方法:

通过指数式丰富 (SELEX) 的联体的系统演化被用来识别新的体.
根据亲和力和特异性选择了表现最好的阿胺体,ATP18-13.
使用ATP18-13构建了一个光信标传感器,用于ATP检测.

主要成果:

新型体ATP18-13与现有的体相比,对ATP的亲和力显著更高.
ATP18-13表现出优越的特异性,有效地区分腺二酸盐 (ADP),腺单酸盐 (AMP) 和腺.
开发的光灯传感器在缓冲器中达到125nm ATP和细胞培养基中达到1μm的检测极限.
在DNA聚合酶活性期间对脱氧ATP消耗的实时监测被成功证明.

结论:

新发现的DNA体为ATP检测提供了前所未有的亲和力和特异性.
开发的以aptamer为基础的传感器可以对ATP进行敏感和实时监测.
这些体和传感器对各种需要ATP测量的生物应用具有前景.