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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

12.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Annals of surgical oncology·2025
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Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

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Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

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Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

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Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

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相关实验视频

Updated: May 15, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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基于LED的多色扩展分辨率传输光显微镜.

Huaiyuan Zhang1, Yiting Hu1, Xingwei Pu1

  • 1University of Electronic Science and Technology of China, School of Optoelectronic Science and Engineering, Chengdu, China.

Journal of biomedical optics
|April 9, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一个紧的,具有成本效益的多色扩展分辨率光成像系统. 这种创新工具简化了生物样本分析,为研究人员提供了复杂结构的增强可视化.

关键词:
光成像成像技术的使用.发光二极管是一种发光二极管.结构化照明显微镜结构化照明显微镜

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

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相关实验视频

Last Updated: May 15, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 光学显微镜的使用方法
  • 分子生物学分子生物学

背景情况:

  • 光成像为研究复杂生物系统提供了分子特异性.
  • 超分辨率显微镜可以促进生物探索,但通常需要高成本和庞大的设备.
  • 目前的多色超分辨率系统在可访问性和可负担性方面面临限制.

研究的目的:

  • 介绍一款全新的多色扩展分辨率光成像系统.
  • 与现有系统相比,为了简化光学路径,减少尺寸和降低成本.
  • 为生物研究提供可访问的扩展分辨率成像.

主要方法:

  • 使用发光二极管 (LED) 进行简化的光学设计.
  • 在广场显微镜中使用结构化照明与衍射单元.
  • 设计为一个紧的附加单元,用于集成到标准的广场显微镜中.

主要成果:

  • 实现了三色微球珠的并发扩展分辨率成像.
  • 成功展示了生物组织的多色扩展分辨率光成像.
  • 在生物样本中揭示了复杂的结构细节,具有增强的分辨率.

结论:

  • 呈现了一个结构简单且具有成本效益的替代传统超高分辨率显微镜.
  • 提供灵活的多色扩展分辨率光成像功能.
  • 在多式成像和先进的生物研究中具有潜在的应用.