从随机选择的寡核酸中组装催化复合物.
1Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA.
Science advances
|April 30, 2025
概括
早期的生命依赖于催化RNA (核糖酶). 这项研究表明,这些核酶可以从短核酸碎片和DNA辅因子中形成,甚至从随机序列中形成,证明了早期催化剂出现的关键原理.
科学领域:
- 生命起源研究研究生命的起源.
- 生物化学 生物化学
- 分子进化的分子进化.
背景情况:
- 早期生命的功能取决于催化RNA,称为 ribozymes.
- 从原始环境中的更简单的组件组装这些早期催化剂是生命起源研究的一个关键问题.
研究的目的:
- 为了研究催化RNA是否可以在随机序列环境中从多个短核酸碎片组装在一起.
- 为了确定DNA寡合体是否可以作为早期RNA催化剂的重要辅因子.
主要方法:
- 在体外选择实验中使用短RNA库和256种不同的DNA20核酸寡合体.
- 高通量测序用于分析选定的RNA序列.
- 生物化学测试以确认催化活性和辅因子要求.
主要成果:
- 大多数选择的RNA序列 (共1331个) 需要至少一个DNA辅因子来产生活性.
- 四个不同的RNA序列集群显示了依赖DNA辅因子的催化活性.
- 当DNA辅因子随机生成时,这些依赖DNA的RNA催化剂仍然活跃,这表明强大的辅因子招募.
结论:
- 催化RNA-DNA复合体甚至可以在随机DNA序列中形成和运行,这表明早期催化剂进化的合理机制.
- 从随机库中招募寡核酸辅因子是最早的寡核酸催化剂出现的一个重要原则.
- 这项研究提供了关于如何复杂的催化功能可能已经从简单的核酸前体在前生物环境中产生的见解.
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