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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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相关实验视频

Updated: May 16, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

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将Riboglow-FLIM工具箱扩展到不同的光终生产生RNA标签.

Zachary Stickelman1, Nadia Sarfraz1, Morgan K Rice1

  • 1Department of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.

Biochemistry
|May 15, 2025
PubMed
概括
此摘要是机器生成的。

研究人员通过开发九个新的RNA标签来扩展Riboglow-FLIM平台. 这一进步使得使用光终身成像显微镜 (FLIM) 能够同时跟踪活细胞中的多个RNA.

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

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相关实验视频

Last Updated: May 16, 2025

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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

485

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • RNAs的细胞下定位对于它们的生物功能至关重要.
  • 基因编码的RNA标签与光探针相结合,可以可视化RNA动态.
  • 里博格洛-FLIM平台之前已经证明了RNA标记和光寿命测量的概念验证.

研究的目的:

  • 为Riboglow-FLIM平台系统地扩大RNA标签序列空间.
  • 为了评估不同 рибо交换机衍生RNA序列的多重复合能力.
  • 为在活细胞中同时追踪多个RNA建立基础.

主要方法:

  • 评估各种RNA序列,以扩展Riboglow-FLIM标签库.
  • 采用了以文献为导向的方法,包括对源自 ribo-switch 的标签的遗传学分析.
  • 采用基于已建立的Riboglow RNA标签的理性设计方法来创建新的变体.

主要成果:

  • 描述了九个不同的RNA标签,证明了广泛的光寿命.
  • 通过观察每个标签的独特光寿命签名,证实了复合的潜力.
  • 建立了同时分化和跟踪多个RNA分子的基础.

结论:

  • 开发的RNA标签显著提高了Riboglow-FLIM平台的多重复合能力.
  • 这项工作为在活生物系统中同时进行RNA成像提供了一个强大的工具包.
  • 这些发现为对RNA的时空动态和功能进行先进研究铺平了道路.