Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

56.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CRISPR-Cas9 trans-cleavage is hindered by a flanked R-loop, an elongated spacer, and an inactive HNH domain.

Nature communications·2026
Same author

A solar panel-origin microalga, Coelastrella thermophila D14, with high potential for wastewater biotechnology.

Applied microbiology and biotechnology·2025
Same author

Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner.

Journal of experimental botany·2025
Same author

Virus Detection by CRISPR-Cas9-Mediated Strand Displacement in a Lateral Flow Assay.

ACS applied bio materials·2025
Same author

Regulatory Effects of RNA-Protein Interactions Revealed by Reporter Assays of Bacteria Grown on Solid Media.

Biosensors·2025
Same author

Probing the orthogonality and robustness of the mammalian RNA-binding protein Musashi-1 in Escherichia coli.

Journal of biological engineering·2024
Same journal

Engineering a Cytochrome P450 <i>O</i>-Demethylase for the Bioconversion of Hardwood Lignin.

ACS synthetic biology·2026
Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026
Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026
Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026
Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026
Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026
查看所有相关文章

相关实验视频

Updated: May 20, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K

将蓝光激活的T7RNA聚合酶系统数字化,使用一个控制的肋骨调节器.

Sara Baldanta1, Guillermo Rodrigo1

  • 1Institute for Integrative Systems Biology (I2SysBio), CSIC - University of Valencia, 46980 Paterna, Spain.

ACS synthetic biology
|May 19, 2025
PubMed
概括
此摘要是机器生成的。

我们使用一种新的肋骨调节模块改进了光控制的基因表达系统. 这种增强的光遗传系统显著提高了动态范围,以获得更好的生物技术应用.

关键词:
抗生素耐药性 抗生素耐药性视觉遗传学 视觉遗传学小的RNA小的RNA小的RNA合成生物学 合成生物学

更多相关视频

Rapid Characterization of Genetic Parts with Cell-Free Systems
05:00

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

1.8K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

669

相关实验视频

Last Updated: May 20, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K
Rapid Characterization of Genetic Parts with Cell-Free Systems
05:00

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

1.8K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

669

科学领域:

  • 合成生物学 合成生物学
  • 视觉遗传学 视觉遗传学
  • 分子工程分子工程分子工程

背景情况:

  • 光遗传系统提供精确的基因表达控制,但由于黑暗活动而受到有限的动态范围的影响.
  • 现有的系统需要改进,以便在生物技术中得到更广泛的应用.

研究的目的:

  • 通过改善其动态范围和减少泄漏表达来增强光遗传系统.
  • 设计一种新的光控制基因表达系统,并将输出数字化.

主要方法:

  • 开发了一种分裂的T7RNA聚合酶系统,与蓝光可诱导磁体融合.
  • 包含一个对无水三环素和合成小RNA敏感的四环素控制的肋骨调节模块.
  • 在聚合酶片段翻译上实施了压制机制,由蓝光缓解.

主要成果:

  • 在暴露在蓝光下,动态范围提高了13倍.
  • 在经过化学诱导预先适应的细胞中,进一步增强动态范围,达到23倍.
  • 在细菌中通过光控制的抗生素耐药性证明了功能应用.

结论:

  • 工程制造的肋骨调节模块有效地将光控制基因表达数字化.
  • 监管层的整合为开发改进的光遗传电路提供了一个强大的战略.
  • 这种方法推进了需要精确基因调节的基于光的生物技术应用.