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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.4K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.9K

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相关实验视频

Updated: Sep 19, 2025

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

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无标签的免疫组织化学 通过斜率发射反射率差异成像.

Meng Li1, Yuanyuan Xie1, Dandan Ji1

  • 1State Key Laboratory of Resource Insects; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education; School of Materials and Energy, Southwest University, Chongqing 400715, P. R. China.

Analytical chemistry
|June 5, 2025
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概括

这项研究引入了一种新的无标签免疫组织化学 (IHC) 方法,使用斜发射反射率差异 (OIRD) 成像. 这种技术可以在没有标记抗体的情况下绘制生物标记物分布图,从而推进病理分析和疾病诊断.

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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
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Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

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相关实验视频

Last Updated: Sep 19, 2025

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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
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Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

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科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 病理学 病理学 病理学
  • 生物化学 生物化学

背景情况:

  • 免疫组织化学 (IHC) 是病理学的标准技术,用于可视化组织中的生物标志物.
  • 传统的IHC依赖于标记的抗体,这可能是昂贵和耗时的.
  • 需要更有效,更容易获得的生物标志物检测方法.

研究的目的:

  • 开发和验证一个无标签的IHC成像策略.
  • 为了证明斜发射反射率差异 (OIRD) 成像用于生物标志物映射的能力.
  • 评估这种方法在病理分析和药物查方面的潜力.

主要方法:

  • 使用了斜发射反射率差异 (OIRD) 成像技术.
  • 在组织切片上应用OIRD,用于无标签的生物标志物可视化.
  • 监控的动态抗体结合动力学.
  • 与传统光IHC相比验证的结果.

主要成果:

  • 在没有标记抗体的情况下,成功地绘制了生物标志物的空间分布.
  • 在无标签的IHC中证明了OIRD成像的有效性.
  • 观察和监测的抗体与组织的结合动力学.
  • 通过与光IHC进行比较,证实了该策略的有效性.

结论:

  • 通过OIRD引入了一种开创性的无标签IHC成像策略.
  • 这种方法避免了需要标记的抗体,简化了这个过程.
  • OIRD技术为病理分析,疾病诊断和药物查提供了一个有前途的工具.