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相关概念视频

The Tumor Microenvironment02:17

The Tumor Microenvironment

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

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Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
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Structures of Solids02:22

Structures of Solids

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Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
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Tumor Immunotherapy01:27

Tumor Immunotherapy

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Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
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Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

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Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
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Tumor Progression02:07

Tumor Progression

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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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相关实验视频

Updated: Jan 29, 2026

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
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三维瘤球体揭示了B7-H3 CAR T细胞透动力学和微环境诱导的功能重编程在固体瘤中.

Feng Chen1,2, Ke Ning1, Yuanyuan Xie1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Materials and Energy, Southwest University, Chongqing 400715, China.

Cells
|January 28, 2026
PubMed
概括

这项研究引入了一种3D瘤球形模型,以克服评估化学抗原受体 (CAR) T细胞治疗固体瘤的局限性. 该平台有效地建模瘤微环境 (TME),并评估CAR T细胞对癌症球体的疗效.

关键词:
三维瘤球形体B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3 B7-H3在CAR T细胞中,透T细胞的透情况免疫疗法 免疫疗法瘤微环境是一个微环境.

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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
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相关实验视频

Last Updated: Jan 29, 2026

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Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
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科学领域:

  • 免疫学 免疫学 免疫学
  • 在瘤学瘤学.
  • 生物技术是生物技术.

背景情况:

  • 化学抗原受体 (CAR) T细胞疗法在血液癌症中表现有前途,但由于瘤微环境 (TME),在固体瘤中面临挑战.
  • 现有的二维细胞培养模型无法准确地表示复杂的TME,限制了对CAR T细胞治疗在固体瘤中的疗效的评估.

研究的目的:

  • 验证3D瘤球形平台,用于评估固体瘤中CAR T细胞治疗.
  • 评估TME对CAR T细胞功能和疗效的影响,使用一种新的3D模型.

主要方法:

  • 开发了一个3D瘤球形模型,使用糖微波系统从固体瘤细胞系产生均的B7-H3-阳性球形.
  • 在3D球形中通过流细胞计分析了TME相关的免疫调制 (B7-H3,MHC I/II,APM).
  • 与B7-H3 CAR T细胞共同培养的球体来评估细胞毒性,瘤活力以及CAR T细胞激活,耗尽和细胞因子的产生.

主要成果:

  • 3D平台成功地从前列腺癌 (DU 145) 和乳腺癌 (SUM159) 细胞系生成了B7-H3阳性球体.
  • 3D球体表现出类似TME的免疫逃避特征,降低MHC-I和抗原处理机器 (APM) 的调节,同时提高MHC-II和calreticulin.
  • 卡尔-T细胞有效地透到球体中,表现出细胞毒性,结构性破坏,以及增强的激活,耗尽和效应器功能,包括增加IFN-γ和TNF-α的产生.

结论:

  • 经过验证的3D瘤球形平台有效地回顾了与CAR T细胞治疗相关的关键TME约束.
  • 这种3D模型提供了一种可行且具有成本效益的临床前工具,用于评估固体瘤中CAR T细胞疗法,其性能优于传统的2D分析.