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温度适应性驱动功能多样性和微生物群落内的横向基因转移在达克固态发酵中的Daqu固态发酵中
Yi Luo1, Hui Liao1, Liming Wu2
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
Bioresource technology
|June 5, 2025
概括
高温达克 (HTD) 发酵涉及温度依赖的微生物变化,影响风味和效率. 温度压力驱动基因转移,影响微生物化和HTD质量.
科学领域:
- 微生物学 微生物学
- 发酵科学 发酵科学
- 食品科学 食品科学 食品科学
背景情况:
- 高温达克 (HTD) 固态发酵对于高效发酵至关重要,但其温度依赖的微生物动力学仍然不清楚.
- 在不同温度下了解微生物组合,化和代谢概况对于优化HTD生产至关重要.
研究的目的:
- 研究温度适应性对微生物群落,风味特征和代谢网络在三个不同的HTDs的影响.
- 分析横向基因转移 (HGT) 事件及其与HTD中温度适应和风味代谢的关联.
主要方法:
- 使用MetaCHIP分析挥发性化合物,微生物社区结构 (细菌和真菌),代谢网络和HGT事件.
- 对三种HTD (伦舒,山,毛元) 的比较分析,以确定微生物特征,味道和样品接近之间的相关性.
主要成果:
- 确定了125种挥发性化合物,其中包括特定的pyrazines,酸和酒精作为关键风味物质.
- 揭示了共享的主导细菌系 (Bacillus, Kroppenstedtia) 和真菌系 (Paecilomyces, Aspergillus, Rasamsonia, Lichtheimia) 的共同的主导性.
- 证明了风味代谢,微生物结构和HGT事件与样本遗传学距离之间的强烈相关性,HGT与高温适应性和特征性风味有关.
结论:
- 温度压力显著影响微生物调节和适应性基因转移在堆叠发酵HTDs.
- 微生物的结构和功能特征与样本接近度的下降趋同,表明局部适应.
- 这些发现为HTD质量分类和通过微生物化优化固态发酵效率提供了关键的见解.
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