Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cohesins02:20

Cohesins

4.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.4K
DNA Topoisomerases02:02

DNA Topoisomerases

31.1K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.1K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.0K
DNA Helicases00:55

DNA Helicases

21.2K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.2K
Condensins02:15

Condensins

3.4K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.4K
The DNA Replication Fork01:02

The DNA Replication Fork

35.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Devising a divisome for synthetic cells.

Nature reviews. Chemistry·2026
Same author

A modular framework for automated segmentation and analysis of AFM imaging of chromatin organization.

Nucleic acids research·2026
Same author

PDS5 proteins control genome architecture by limiting the lifetime of cohesin-NIPBL complexes.

Molecular cell·2026
Same author

Terminal Conjugation Enables Nanopore Sequencing of Peptides.

Journal of the American Chemical Society·2026
Same author

Chromatin boundary permeability is controlled by CTCF conformational ensembles.

bioRxiv : the preprint server for biology·2025
Same author

Two CTCF motifs impede cohesin-mediated DNA loop extrusion.

Molecular cell·2025

相关实验视频

Updated: Jun 16, 2025

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.4K

在循环挤出过程中,凝聚性超级卷DNA在循环挤出过程中.

Iain F Davidson1, Roman Barth2, Kota Nagasaka1

  • 1Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria.

Cell reports
|June 14, 2025
PubMed
概括

凝聚蛋白 (一种蛋白质复合体) 在循环挤出过程中负面地超绕DNA,将其扭转在链和之间. 这种超级卷圈对于形成和维护基因组架构至关重要.

关键词:
科普:分子生物学 分子生物学在SMC的综合体中,凝聚力 在凝聚力中循环挤出循环的挤出.超级卷圈的超级卷圈是一种超级卷圈.这种类型的 topoisomeraseses.

更多相关视频

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
12:19

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Published on: November 10, 2016

8.3K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

985

相关实验视频

Last Updated: Jun 16, 2025

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.4K
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
12:19

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Published on: November 10, 2016

8.3K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

985

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 凝聚素对于将基因组DNA组织成循环至关重要.
  • 这些循环影响染色体组合,基因调节和重组.
  • 在循环挤出过程中,凝聚素介导的DNA转位的确切机制尚不清楚.

研究的目的:

  • 为了研究凝聚素挤出DNA循环的生物物理机制.
  • 为了确定DNA超级卷是否参与凝聚的循环挤出过程.
  • 为了阐明DNA超级卷在基因组架构中的作用.

主要方法:

  • 在体外生化测试研究凝聚素-DNA相互作用.
  • 对ATPase活性或DNA结合有缺陷的凝聚素突变体的分析.
  • 细胞测试以评估循环形成和基因组结构.
  • 耗尽topoisomerase I以研究其对循环挤出的影响.

主要成果:

  • 在循环挤出过程中,凝聚素在DNA中积极产生负超卷.
  • 这种超级卷曲取决于凝聚素的ATPase头和其链上的DNA结合部位的参与.
  • 一种在超级卷结中受损的凝聚性突变会在细胞中产生更短的DNA循环.
  • 拓聚酶I的耗尽也导致循环缩短,这表明DNA放松的作用.

结论:

  • 负DNA超卷是凝聚性循环挤出机制的组成部分.
  • 凝聚素对DNA的扭曲对于其在基因组组织中的功能至关重要.
  • 通过诸如拓聚酶I之类的因素来放松超绕的DNA是有效的凝聚蛋白介导循环挤出和保持基因组结构所必需的.