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相关概念视频

CRISPR01:59

CRISPR

53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

375
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
375
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

138
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
138
Homologous Recombination02:31

Homologous Recombination

52.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.7K

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相关实验视频

Updated: Sep 18, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

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基于CRISPR/Cas12a系统的传感器中的分离技术.

Xianmin Ding1, Xueying Lei2, Songcheng Yu2

  • 1Department of Nuclear Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, China.

Biotechnology advances
|June 20, 2025
PubMed
概括
此摘要是机器生成的。

分裂技术增强了CRISPR/Cas12a核酸分析系统,使得RNA等新目标的检测成为可能,并扩大了先进生物传感器的可编程性.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas12a.传感器 传感器 传感器拆分 分裂 拆分 分裂

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 生物传感器技术技术

背景情况:

  • CRISPR/Cas12a系统是核酸分析的强大工具,以其特异性,灵敏性和可编程性而闻名.
  • 最近的进展涉及将分割技术应用于CRISPR/Cas12a系统的各种组件,包括激活器,crRNA,记者和Cas12a.

研究的目的:

  • 审查基于CRISPR/Cas12a的传感器中分割技术的进展.
  • 总结一下用于CRISPR/Cas12a系统的分割技术的好处.
  • 讨论这些传感器的挑战和未来前景.

主要方法:

  • 对用于CRISPR/Cas12a系统的分割技术的文献综述.
  • 分析分离组件如何使新目标的检测成为可能.
  • 评估对传感器成本,稳定性和可编程性的影响.

主要成果:

  • 分裂技术扩大了CRISPR/Cas12a系统的检测能力,包括没有PAM的dsDNA,短ssDNA (<15nt) 和RNA.
  • 它允许开发无标签,低成本的传感器.
  • 分解技术可方便构建具有多个输入和输出的蜂逻辑电路.

结论:

  • 分裂技术通过扩大目标范围,提高crRNA稳定性,增加战略可编程性和降低检测成本,显著增强了CRISPR/Cas12a系统.
  • 这些进步为更具多功能性和成本效益的核酸检测平台铺平了道路.