Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

60.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
60.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Navigating Human Epidermal Growth Factor Receptor 2 (HER2) Assessment by Organ Systems: A Guidebook to HER2 Testing in Solid Tumors.

Archives of pathology & laboratory medicine·2026
Same author

Comparison of the somatic mutations in breast carcinomas in sporadic and BRCA1 carrier patients through targeted next generation sequencing.

Human pathology·2026
Same author

Utility of Fine-Needle Aspiration Cytology Smears for Molecular Analysis of Lung Adenocarcinomas: A Comparative Analysis of Next-Generation Sequencing Quality Metrics.

Diagnostic cytopathology·2026
Same author

Clinical significance of quantification of perineural cancer invasion on MRI-targeted prostate biopsy.

Diagnostic pathology·2025
Same author

Unusual Morphologic Presentation of Perineural Spread From Cutaneous Squamous Cell Carcinoma: Diagnosis Aided by Comprehensive Molecular Analysis and Machine Learning.

Journal of cutaneous pathology·2025
Same author

Estrogen receptor-low positive breast cancer: Historical perspective and recent advancements.

Human pathology·2025

相关实验视频

Updated: Sep 18, 2025

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K

一个修改的FLT3PCR试验,使用磁带站读出.

Elizabeth Adele Blake1, Madhurya Ramineni1, Zoltán N Oltvai1

  • 1Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14627, USA.

Genes
|June 26, 2025
PubMed
概括

使用TapeStation 4200平台进行修改的FLT3 PCR测定为检测急性髓性白血病 (AML) 中FLT3突变提供了快速而准确的替代方案. 这种方法对较大的内部并列重复 (ITD) 和氨酸激酶域 (TKD) 突变具有高度敏感性和特异性,补充了下一代测序 (NGS) 工作流.

关键词:
在FLT3-ITDD中使用.在FLT3-TKD中.屏幕录像带测试方法录音机 录音机 录音机自动化的凝电泳.毛细血管电泳 毛细血管电泳一个电光电场图 (electropherogram) 是一个电光电场图.大型ITD是一个大型ITD.在PCR后进行碎片分析.非常短的插件插件.

更多相关视频

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

10.5K
Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
06:38

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Published on: May 3, 2019

8.9K

相关实验视频

Last Updated: Sep 18, 2025

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

10.5K
Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
06:38

Droplet Digital TRAP ddTRAP: Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Published on: May 3, 2019

8.9K

科学领域:

  • 分子诊断学 分子诊断
  • 在瘤学瘤学.
  • 遗传学 遗传学 是一个

背景情况:

  • 在诊断和管理急性髓性白血病 (AML) 时,FLT3突变测试至关重要.
  • 目前的标准FLT3PCR与毛细管电泳 (CE) 是有效的,但耗时且技术要求很高.
  • 需要更快,更有效的替代品来取代FLT3突变分析的传统CE.

研究的目的:

  • 为了验证使用Agilent 4200 TapeStation平台快速读取结果的修改后的FLT3 PCR试验.
  • 评估TapeStation 4200用于检测FLT3-ITD和TKD突变的灵敏度,特异性和可重复性.
  • 将TapeStation 4200测试的性能与NGS和PAGE等既有方法进行比较.

主要方法:

  • 使用Agilent 4200 TapeStation进行碎片分析,开发了一种修改后的FLT3 PCR测定.
  • 该试验使用22例患者样本用于FLT3-ITD和18例用于FLT3-TKD突变进行了验证.
  • 结果与先前验证的下一代测序 (NGS) 和聚烯胺凝电泳 (PAGE) 方法进行了比较.

主要成果:

  • 磁带Station 4200在检测FLT3-ITD (>15bp) 和TKD突变方面表现出100%的灵敏度,特异性和高可重复性.
  • 对于可检测的突变,与NGS和PAGE方法的一致性接近100%.
  • 发现的一个关键限制是无法可靠地检测小于15bp的FLT3-ITD.

结论:

  • 基于TapeStation 4200的FLT3 PCR测定是一种合适的伴侣诊断工具,可与NGS平台一起进行快速分析.
  • 它可以有效地检测较大的FLT3-ITD,并通过NGS生物信息算法解决潜在的错误调用或下调.
  • 由于无法检测非常短的FLT3-ITDs,因此不建议将该试验作为独立的诊断工具.