Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Label-free high-resolution volume imaging of cleared testes.

Reproduction (Cambridge, England)·2026
Same author

Light as a feather: A novel approach for whole- feather microstructure imaging through tissue clearing and autofluorescence imaging.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026
Same author

Label-free, High-resolution Fluorescence Imaging of the Intestinal Wall by Image Scanning and Confocal Microscopy.

Gastro hep advances·2025
Same author

Imaging the enteric nervous system.

Frontiers in neuroanatomy·2025
Same author

A first morphological and electrophysiological characterization of Fañanas cells of the mouse cerebellum.

The Journal of physiology·2025
Same author

[The gut, a whistleblower, in the early stages of Parkinson's disease].

Medecine sciences : M/S·2024

相关实验视频

Updated: Jun 28, 2026

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
07:46

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Published on: December 31, 2016

12.4K

快速组织清除和体积成像技术用于解剖学.

Clément Ricard1, Doriane Hazart1, Brigitte Delhomme1

  • 1Université Paris Cité, SPPIN, Saint-Pères Paris Institute for the Neurosciences, CNRS, Paris, France.

Microscopy research and technique
|June 28, 2025
PubMed
概括

显微镜现在使用先进的组织清除和3D显微镜技术在3D中成像整个器官,克服了详细解剖研究传统2D方法的局限性.

科学领域:

  • 生物成像技术 生物成像技术
  • 细胞生物学 细胞生物学
  • 解剖病理学 解剖病理学

背景情况:

  • 传统的光学显微镜仅限于薄样本,阻碍了3D分析.
  • 立体学从二维切片提供3D解释,但很费力,容易出错.
  • 需要取得进展,以三维成像完整的生物组织.

研究的目的:

  • 审查现代组织清除方法.
  • 描述先进的3D显微镜技术.
  • 突出结合的方法用于完整组织的亚细胞成像.

主要方法:

  • 组织清除技术,使样品变得透明.
  • 先进的显微镜,包括共焦,双光子和光片显微镜.
  • 整合清除和3D成像,用于完整的器官分析.

主要成果:

  • 组织清除使以前不透明的样本能够进行深度成像.
  • 3D显微镜技术可以实现显著的成像深度.
  • 组合方法允许在清除的整个器官中进行亚细胞分离.

结论:

关键词:
解剖学和病理学 解剖学和病理学人体解剖学 解剖学 解剖学光片显微镜光片显微镜三维显微镜三维显微镜组织清除 组织清除两个光子显微镜的显微镜

更多相关视频

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
06:18

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

10.6K
Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies
08:49

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

338

相关实验视频

Last Updated: Jun 28, 2026

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
07:46

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Published on: December 31, 2016

12.4K
Optical Clearing and Imaging of Immunolabeled Kidney Tissue
06:18

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

10.6K
Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies
08:49

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

338
  • 现代显微镜已经克服了生物样本的3D成像边界.
  • 组织清除和3D显微镜为解剖学和病理学提供了新的视角.
  • 这些综合技术有助于对完整器官进行详细的亚细胞分析.