Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Meta-analysis of DNA methylation aging signatures in 17 human tissues.

Nature aging·2026

Same author

Guidelines on optimizing DNA methylation reference panels for cell-type deconvolution.

Communications biology·2026

Same author

Variations in Innate Immune Cell Subtypes Correlate with Epigenetic Clocks, Inflammaging and Health Outcomes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Epigenetic clocks and inflammaging: pitfalls caused by ignoring cell-type heterogeneity.

GeroScience·2025

Same author

Epigenetic ageing clocks: statistical methods and emerging computational challenges.

Nature reviews. Genetics·2025

Same author

Integrative analysis of genomic and epigenomic regulation reveals miRNA mediated tumor heterogeneity and immune evasion in lower grade glioma.

Communications biology·2024

Search research articles

相关实验视频

Updated: Sep 17, 2025

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

一个改进的参考库和方法,用于准确的细胞类型解卷批量组织miRNA数据.

Shaoying Zhu¹, Hui Yang², Jun Liu³

¹Center for Medical Research and Innovation of Pudong Hospital, Fudan University Pudong Medical Center, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Fudan University, 200032, Shanghai, China.

Nature communications

|July 2, 2025

概括

研究人员开发了一种新的计算工具,使用microRNA (miRNA) 表达数据来估计复杂组织中的细胞类型. 这种方法有助于发现癌症等疾病的miRNA生物标志物,并监测免疫反应.

更多相关视频

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

相关实验视频

Last Updated: Sep 17, 2025

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

微RNAs (miRNAs) 在生物过程和疾病中至关重要,显示出作为生物标记物的潜力.
复杂组织中细胞类型的异质性使miRNA生物标记物的识别变得复杂.
目前的单细胞RNA-Seq方法在miRNA量化方面遇到了困难,并且经常缺少用于解卷的匹配数据.

研究的目的:

从散装组织miRNA表达数据中估计细胞类型比例的计算方法的开发.
创建一个新的miRNA表达参考库和解卷工具.
为了解决在复杂的生物样本中分析细胞类型特定的miRNA功能的迫切需要.

主要方法:

开发一个新的miRNA表达参考库.
创建一个用于细胞类型组成估计的计算解卷工具.
在各种组织中验证工具的准确性和稳定性,包括全血和固体组织.

主要成果:

开发的工具准确地从批量miRNA数据中估计了细胞类型的比例.
该方法在各种组织类型中表现出强度.
在查与年龄相关的miRNA和监测传染病中的免疫景观方面成功应用.

结论:

已经建立了一个计算框架,用于从复杂组织中准确解卷miRNA数据.
该工具有助于识别特定于细胞类型的miRNA生物标志物,用于癌症诊断和预后.
这种方法提高了miRNA分析在各种生物和临床环境中的实用性.