Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Organization01:24

Protein Organization

7.3K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
7.3K
Protein Folding01:22

Protein Folding

121.6K
Overview
121.6K
Protein and Protein Structure02:15

Protein and Protein Structure

81.5K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
81.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Induced alanine auxotrophy as a therapeutic strategy against <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Chirality transfer from chiral perovskite to molecular dopants via charge transfer states.

Nature communications·2026
Same author

Octahedral Coordination Cages of Co(II) and Ni(II): Enhanced Probe Signal Through Symmetry.

Inorganic chemistry·2026
Same author

Synthesis of Tetra-Porphyrin Tubes for Catalysis and Host-Guest Chemistry.

Inorganic chemistry·2026
Same author

Direct from the seed: an atomic resolution protein structure by ab initio MicroED.

Nature communications·2026
Same author

Strain-Responsive and Self-Healing Chiral Polymer Vitrimer Composites: Tunable Circular Dichroism and Mechanochromic Recovery in Self-Standing Films.

ACS applied materials & interfaces·2026
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Sep 16, 2025

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.2K

直接从种子:一个原子分辨率蛋白质结构由 Ab Initio MicroED

Purna Chandra Rao Vasireddy1, Timothy Low-Beer1, Katherine A Spoth2

  • 1Department of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14203.

bioRxiv : the preprint server for biology
|July 9, 2025
PubMed
概括
此摘要是机器生成的。

研究人员发现,在净化过程中形成的蛋白质纳米晶是微晶电子衍射 (MicroED) 的理想选择. 这种方法实现了创纪录的0.85 Å分辨率,使自动化模型构建和原子分辨率成为可能.

关键词:
原子分辨率的原子分辨率化电磁波是一种冷电磁波.微晶的电子 difraktion 是一种微晶的电子 difraktion.自然产品 自然产品阶段化是分阶段进行的.

更多相关视频

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

12.5K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

12.8K

相关实验视频

Last Updated: Sep 16, 2025

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.2K
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

12.5K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

12.8K

科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学

背景情况:

  • 传统的X射线晶体学在某些蛋白质样本上面临局限性.
  • 蛋白质净化可以产生意想不到的晶体形式.

研究的目的:

  • 探索自发形成的蛋白质纳米晶体对于高分辨率结构确定的实用性.
  • 建立一种新的工作流程,用于从原始生物样本中解决蛋白质结构.

主要方法:

  • 在克兰宾净化过程中蛋白质纳米晶体的自发形成.
  • 使用微晶电子衍射 (MicroED) 的高通量数据收集.
  • Ab initio结构解决方案和自动化模型构建.

主要成果:

  • 从纳米晶体中实现了创纪录的0.85 Å分辨率的蛋白质结构.
  • 在58个纳米晶体中获得了高相关系数 (>99%).
  • 启用了自动化模型构建,并解决了单个原子.

结论:

  • 自发形成的蛋白质纳米晶体适用于MicroED.
  • 这个工作流提供了一个可扩展的管道,用于原子级蛋白质结构的确定.
  • 能够对以前难以处理的生物点进行结构分析.