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Updated: Sep 16, 2025

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

全长转录组测序揭示了Treg特定的异形表达在激活时

Yohei Sato^1,2, Erika Osada², Yoshinobu Manome²

¹Project Research Unit, Laboratory of Immune Cell Therapy, The Jikei University School of Medicine, Tokyo 105-8461, Japan.

International journal of molecular sciences

|July 12, 2025

概括

调节性T细胞 (Tregs) 呈现出独特的异型组合,包括一个独特的FOXP3异型,这对免疫系统调节至关重要. 这种Treg特异性特征是在T细胞受体刺激后显现的.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

调节FOXP3+的T细胞 (Tregs) 对免疫平衡至关重要.
人类Tregs独特地表达了一种缺少2 (三角形) 异构体的FOXP3异构体.
假设Tregs具有独特的基因和异型表达特征.

研究的目的:

识别和确认Treg特定的异型组.
调查异形表达在Treg功能中的作用.
分析Tregs与常规T细胞 (Tconvs) 之间的异型使用差异.

主要方法:

长读高通量异形序列化 (Iso-seq) 用于全基因组异形使用分析.
在Tregs和Tconvs之间比较异型表达特征.
T细胞受体 (TCR) 刺激试验和PD-L1抗体阻断实验.

主要成果:

通过使用Iso-seq.确定了一个独特的Treg-特定的异型谱.
与Tconvs.不同的是,Tregs优先表达全长FOXP3异型,与Tconvs.不同.
激活的Tregs,但不是Tconvs或非激活的Tregs,在TCR刺激时显示了ICOS和PD-L1的优先表达.
PD-L1阻断降低了Treg抑制功能,但没有影响FOXP3表达.

结论:

Tregs具有独特的异构体谱,包括特定的FOXP3异构体.
这种独特的曲目在多克隆TCR刺激后变得更加明显.
异形表达在Treg功能和免疫调节中起着重要作用.

关键词:

这是FOXP3P3的.在ISO-seqq中使用.在PD-L1中.在RNA-seqqq.监管性T细胞 (Tregs) 是一种

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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

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