Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SenSet defines cell-type specific senescence signatures in the aged human lung.

The EMBO journal·2026
Same author

MolQuery: Prediction of Lipid Synthesizability Using Active Learning.

ACS omega·2026
Same author

Deep Batch Active Learning for Protein Structure Modeling.

Journal of computational biology : a journal of computational molecular cell biology·2026
Same author

Evaluation of statistical differential analysis methods for identification of senescent cells using single-cell transcriptomics.

Cell reports methods·2026
Same author

Single-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy.

Nature communications·2026
Same author

Toward Artificial Intelligence in Oncology and Cardiology: A Narrative Review of Systems, Challenges, and Opportunities.

Journal of clinical medicine·2025

相关实验视频

Updated: Sep 15, 2025

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
10:45

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Published on: May 29, 2017

10.0K

从单细胞数据恢复时间变化的网络.

Euxhen Hasanaj1, Barnabás Póczos1, Ziv Bar-Joseph1,2

  • 1Machine Learning Department, Carnegie Mellon University, Pittsburgh, PA 15213, United States.

Bioinformatics (Oxford, England)
|July 15, 2025
PubMed
概括

我们开发了Marlene,这是一个深度学习工具,可以从单细胞数据中重建动态基因调节网络. 这种方法准确地识别了复杂的生物过程中的基因相互作用,如衰老和免疫反应.

科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 系统生物学 系统生物学

背景情况:

  • 基因调节对于发育和疾病至关重要.
  • 传统方法难以处理大量的时间序列单细胞数据.
  • 需要新的方法来准确地重建时间基因网络.

研究的目的:

  • 开发一种深度学习模型来推断动态基因调节网络.
  • 为了应对时间序列单细胞基因表达数据所带来的挑战.
  • 为了能够识别动态生物过程中的基因相互作用.

主要方法:

  • 开发了Marlene,一个使用自我注意机制的深度神经网络.
  • 嵌入的循环单位,用于随时间变化的网络权重.
  • 采用超级学习来提高准确性,特别是对于罕见的细胞类型.

主要成果:

  • 玛琳成功地从时间序列单细胞数据中推断出动态基因网络.
  • 该模型准确地重建时间网络,即使对于罕见的细胞群体.
  • 确定了与COVID-19免疫反应,纤维化和衰老相关的基因相互作用.

结论:

更多相关视频

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
10:21

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

6.2K
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

3.0K

相关实验视频

Last Updated: Sep 15, 2025

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
10:45

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Published on: May 29, 2017

10.0K
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
10:21

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

6.2K
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

3.0K
  • 马琳为重建动态基因调节网络提供了一种强大的新方法.
  • 该工具有助于发现驱动特定生物反应的基因相互作用.
  • 这项工作为针对复杂疾病的新型治疗策略铺平了道路.