Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

15.2K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
15.2K
Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

2.6K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
2.6K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.4K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.4K
Mitochondrial Membranes01:45

Mitochondrial Membranes

12.4K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
12.4K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.3K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Long-Term Salt Exposure Reprograms the Nicotiana tabacum BY-2 Suspension Proteome and Metabolome Toward Stabilization of the Core Metabolic Pathways, Protein Turnover Machinery Modifications, and Protective Metabolome Adjustments.

Plant, cell & environment·2026
Same author

IGF2BPs directly regulate the noncanonical translation of toxic proteins from mutant FMR1 mRNA containing expanded CGG repeats.

Nature communications·2025
Same author

Human VDAC3 as a sensor of the intracellular redox state: contribution to cytoprotection mechanisms in oxidative stress.

Biochimica et biophysica acta. Bioenergetics·2025
Same author

An evaluation of bimekizumab for the treatment of hidradenitis suppurativa.

Expert opinion on biological therapy·2025
Same author

Insufficiency of 40S ribosomal proteins, RPS26 and RPS25, negatively affects biosynthesis of polyglycine-containing proteins in fragile-X associated conditions.

eLife·2025
Same author

Common Disease-Difficult Understanding: Readability Analysis of Superficial Skin Fungal Infections On-Line Materials in European Languages.

Mycoses·2025

相关实验视频

Updated: Sep 14, 2025

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
08:33

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Published on: March 24, 2019

7.6K

在酵母模型中,SARS-CoV-2 主蛋白酶会导致线粒体功能障碍.

Wojciech Grabiński1, Anna Kicińska1, Karolina Funtowicz1

  • 1Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.

Scientific reports
|July 18, 2025
PubMed
概括

酵母中SARS-CoV-2主要蛋白酶 (Mpro) 的表达显著损害了 mitochondrial 的生长和功能. 这项研究强调了酵母线粒体对Mpro的敏感性,为其在复杂生物体中的影响提供了洞察力.

更多相关视频

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
08:33

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

4.5K
A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

2.3K

相关实验视频

Last Updated: Sep 14, 2025

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
08:33

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Published on: March 24, 2019

7.6K
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
08:33

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

4.5K
A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

2.3K

科学领域:

  • 线粒体生物学 线粒体生物学
  • 病毒学 病毒学
  • 分子遗传学 分子遗传学

背景情况:

  • 细粒体 (Saccharomyces cerevisiae) 是线粒体研究的一个关键模型生物,因为它具有遗传可访问性和保存途径.
  • 酵母在没有线粒体的情况下生存的能力有助于研究必要的基因突变.
  • 研究酵母中的病毒蛋白毒性,可以了解宿主-病原体相互作用.

研究的目的:

  • 为了评估SARS-CoV-2主要蛋白酶 (Mpro) 在酵母中的表达的毒性.
  • 在有氧条件下检查Mpro对酵母生长和线粒体功能的影响.

主要方法:

  • 使用Saccharomyces cerevisiae作为一个模型生物体.
  • 在酵母中表达的SARS-CoV-2主要蛋白酶 (Mpro).
  • 在需要依赖于线粒体的有氧代谢的条件下培养酵母.
  • 分析了酵母菌的生长,形态和线粒体功能.

主要成果:

  • 证明Mpro表达对酵母具有高度毒性,显著抑制了其生长.
  • 观察到酵母细胞形态的实质性改变.
  • 证明了线粒体功能显著受损.
  • 线粒体通路对Mpro活动的高度敏感性表明.

结论:

  • 酵母线粒体对SARS-CoV-2 Mpro.特别敏感.
  • Mpro表达对细胞功能构成重大威胁.
  • 这些发现为Mpro对真核生物系统的影响提供了有价值的视角.