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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Sep 13, 2025

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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通过人工智能彻底改变了CRISPR技术.

Min-Gyeong Kim1,2, Min-Ji Go1,2, Seung-Hun Kang3

  • 1Biomedical Research Division, Korea Institute of Science and Technology, Seoul, Republic of Korea.

Experimental & molecular medicine
|July 31, 2025
PubMed
概括

人工智能 (AI) 正通过增强CRISPR技术来彻底改变基因组工程. 人工智能改善了导向RNA的设计,预测了非目标效应,并有助于发现新的CRISPR系统,以获得更安全,更精确的基因疗法.

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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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Last Updated: Sep 13, 2025

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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科学领域:

  • 遗传学和基因组学 遗传学和基因组学
  • 生物技术是生物技术.
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因组工程已经取得了重大进展,CRISPR-Cas系统在准特定DNA区域方面提供了前所未有的精度.
  • 目前的CRISPR工具,包括核酶,基编辑器和主要编辑器,可以实现多种基因修改,但面临着诸如可变编辑结果和非目标效应等挑战.

研究的目的:

  • 探索人工智能 (AI) 在推进CRISPR基因组工程技术中的变革性作用.
  • 要强调人工智能集成如何解决现有的局限性,并增强基于CRISPR的工具的能力.

主要方法:

  • 利用大规模的实验数据集来训练AI算法来预测CRISPR活动和设计指导RNA.
  • 利用人工智能识别和设计新型CRISPR系统和Cas蛋白.
  • 应用人工智能来完善现有的CRISPR编辑方式:核酶,基编辑器和主要编辑器.

主要成果:

  • 人工智能显著提高了引导RNA设计,从而提高了准特异性和效率.
  • 基于人工智能的预测模型有效地识别和减轻意外的目标外突变.
  • 人工智能有助于发现新的CRISPR系统,扩大基因组工程的范围.

结论:

  • 人工智能集成对于克服CRISPR技术当前的挑战至关重要,例如非目标效应和可变编辑效率.
  • 在CRISPR工具中,人工智能驱动的进步为更精确,更高效,更安全的基因疗法铺平了道路.
  • 人工智能和CRISPR之间的协同作用有望加速个性化医学和下一代基因编辑应用的创新.