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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Point and Frameshift Mutations01:30

Point and Frameshift Mutations

88
Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
88
Improving Translational Accuracy02:07

Improving Translational Accuracy

11.9K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
11.9K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

3.3K
3.3K
Termination of Translation01:44

Termination of Translation

25.7K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
25.7K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.8K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.8K

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相关实验视频

Updated: Sep 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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核糖体框架移位检测的统计方法.

Alisa Yurovsky1, Justin Gardin1, Bruce Futcher1

  • 1Stony Brook University, Stony Brook, NY, USA.

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PubMed
概括
此摘要是机器生成的。

科学家们开发了一种新的计算方法,以找到编程的移,这是一个过程,在蛋白质合成过程中,核糖体会移动读取. 这种方法准确地识别了这些事件,有可能揭示了许多未知的替代蛋白质在基因组,如酵母和人类.

关键词:
在RNA-seqqq.翻译 翻译 翻译 翻译有界的概率估计估计.框架移位检测检测框架移位检测核糖体造型分析是指核糖体造型.

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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相关实验视频

Last Updated: Sep 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 核糖体通常在蛋白质合成过程中在固定的三核酸框架中读取信使RNA (mRNA).
  • 编程的框架转移,即核糖体转移读取框架,可以产生替代蛋白质,但很难检测到.
  • 现有的方法在识别细胞基因中的新型编程框架转移事件方面缺乏效率.

研究的目的:

  • 开发一个强大的计算框架,用于识别全基因组编程的移事件.
  • 创建一个模拟器来验证开发算法的准确性和灵敏性.
  • 评估通过编程框架转移产生的未注释的替代蛋白质的潜在流行情况.

主要方法:

  • 对核糖体分析数据进行分析,以寻找mRNA读取周期性的异常.
  • 开发一个统计框架,以检测低速率的编程框架转移.
  • 使用定制的移模拟器对核糖体分析数据进行验证.

主要成果:

  • 开发的算法表现出高预测灵敏度,检索97.4%的模拟移.
  • 该方法成功地确定了酵母菌中所有三种已知的编程框架转移基因.
  • 这些发现表明,在酵母基因组中可能存在大量未注释的替代蛋白质.

结论:

  • 新的统计框架有效地识别了全基因组编程的框架转移.
  • 这种方法可以发现大量以前没有注释的替代蛋白质.
  • 对人类和其他基因组进行进一步的研究是有必要的,以探索编程框架转移的全部范围.