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相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.9K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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相关实验视频

Updated: Sep 11, 2025

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

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通过mRNA的功能近距离.

Breanne M Hatfield1, Chase A Weidmann2, Christina A McCutchin1

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, United States.

Biochemistry
|August 12, 2025
PubMed
概括
此摘要是机器生成的。

这项研究表明,调节性蛋白质可以降解传递 RNA (mRNA),当指导到非常规的网站,而不仅仅是它们的通常结合位置. 这一发现扩大了针对性RNA降解疗法的可能性.

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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

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相关实验视频

Last Updated: Sep 11, 2025

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

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科学领域:

  • 分子生物学分子生物学
  • 基因表达规范 基因表达规范
  • 在RNA生物学,RNA生物学.

背景情况:

  • 在整个mRNA生命周期中,RNA-蛋白相互作用对于调节mRNA翻译和降解至关重要.
  • 针对这些相互作用提供了潜在的治疗策略,特别是对于那些难以药物治疗的蛋白质.
  • 了解这些相互作用的空间约束是开发基于RNA的新疗法的关键.

研究的目的:

  • 通过将调节蛋白引导到mRNA上的非传统位点来调查mRNA降解是否可以诱导.
  • 确定错位特定RNA结合蛋白 (DCP2,ZFP36L2,CNOT7) 对记者mRNA表达的影响.
  • 在mRNA调节中探索穿越空间功能近距离的概念.

主要方法:

  • 一个记者mRNA系统被用来评估基因表达.
  • 三种特定的降解诱导蛋白 (DCP2,ZFP36L2,CNOT7) 被人工引导到各种地点,包括异常位置,穿过记者mRNA.
  • 测量了记者mRNA表达水平的变化,以量化由蛋白质错位诱导的降解.

主要成果:

  • DCP2 (5'分解酶) 只有在针对5'未翻译区域 (UTR) 时才能减少表达.
  • 当针对3'-UTR或编码序列的3'半部分时,ZFP36L2 (3'-UTR适应蛋白) 的表达减少.
  • 当向任何位置时,CNOT7 (CCR4-NOT死亡酶子单元) 的表达减少,在5'-和3'-UTR中产生最强烈的影响.

结论:

  • 通过将调节蛋白向远离它们常规结合点的位置,可以实现mRNA降解.
  • 这项研究表明,mRNA分子之间存在广泛的透过空间的功能性接近.
  • 这些发现对理解大规模RNA结构和推进向RNA降解治疗有重大意义.