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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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相关实验视频

Updated: Sep 10, 2025

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

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电压编程序列光编码 (VPSFE) 允许使用电场图灵图案进行多重复合的现场蛋白质成像

Chen Wang1, Jiayi Zheng1, Zhenghan Xiong1

  • 1Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Phar-maceutical University, Nanjing, 210009, China.

Angewandte Chemie (International ed. in English)
|August 22, 2025
PubMed
概括

我们开发了一种基于电泳的方法, 这种技术消除了洗步骤,使生物研究的多重成像速度更快,更通用.

关键词:
在现场成像多重化蛋白质成像一个单元图灵模式可以免费洗

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Conducting Multiple Imaging Modes with One Fluorescence Microscope

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相关实验视频

Last Updated: Sep 10, 2025

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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科学领域:

  • 分子生物学
  • 生物技术
  • 细胞生物学

背景情况:

  • 基于DNA条形码的免疫标记先进的单细胞蛋白质分析.
  • 传统的多重成像需要缓慢,繁忙的探针移除和混合周期,限制了吞吐量和通用性.

研究的目的:

  • 引入一种基于电泳的现场探针移除方法,用于高通量,特定的多重成像.
  • 开发用于蛋白质检测的电压编程顺序光编码 (VPSFE).
  • 建立基于VPSFE的图灵模式编码策略,用于多重目标识别.

主要方法:

  • 使用低压电泳来去除多余的探头 (2分钟) 和高压电泳来混合探头解离 (3分钟).
  • 通过19轮循环电泳和10轮重复成像验证了强度.
  • 开发了VPSFE用于顺序色彩编码和使用电场进行多重检测的图灵模式策略.

主要成果:

  • 在没有洗步骤的情况下实现高精度的代探头成像.
  • 在19个电泳周期和14个探针中表现出异常的特异性和效率.
  • 成功地将VPSFE应用到与表皮介质转换 (EMT) 相关的蛋白质.
  • 创建了27个独特的光图灵模式, 用3个电压条件和3个光通道编码27个不同的目标.

结论:

  • 基于电泳的方法显著提高了多重现场成像的特异性和效率.
  • 对于复杂的生物样本分析,VPSFE提供了一种快速,通用的,无探针交换的方法.
  • 电场图灵模式编码策略为高密度多重检测提供了一个新平台.