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相关概念视频

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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感知微RNA和内核酶1的 AND-gate DNA 步行器

Zuoling Zhang1,2, Mengling Wang1, Tingting Hu1

  • 1Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, China.

Mikrochimica acta
|August 23, 2025
PubMed
概括

这项研究引入了一个AND-gateDNA步行器,用于同时检测两种癌症生物标志物,即microRNA-21和内核酶1 (FEN1). 这种新型的生物传感器可提高癌症诊断的特异性和灵敏性.

关键词:
其他国家生物传感器一个DNA步行者美国微RNA的使用

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科学领域:

  • 生物技术
  • 分子诊断
  • 纳米技术

背景情况:

  • 精确的癌症诊断依赖于敏感的生物标志物检测.
  • 目前的单一目标检测方法往往缺乏可靠诊断所需的特异性.
  • 同时检测多个生物标志物可以提高诊断准确性.

研究的目的:

  • 开发一种新的AND-gate逻辑DNA步行器,用于同时检测miRNA-21和FEN1.
  • 提高癌症生物标志物检测的特异性和敏感性.
  • 创建多目标生物传感系统的模型.

主要方法:

  • 一个使用金纳米粒子 (AuNPs) 和DNA链的AND门DNA步行系统.
  • 在miRNA-21结合的AuNP上固定捕获链.
  • 用光标记的轨道线进行混合,然后进行FEN1介导的裂变.
  • 检测释放的光信号进行量化.

主要成果:

  • 达到了miRNA-21的23. 3 pM和FEN1的0. 097 pg/μL的检测极限.
  • 在同时检测两种生物标志物方面表现出高灵敏度和特异性.
  • 成功进行miRNA-21和FEN1的细胞内成像.

结论:

  • 开发的AND-gateDNA步行器可以精确和灵敏地同时检测两种癌症生物标志物.
  • 这种系统为癌症诊断和了解疾病机制提供了重大进步.
  • DNA步行器是开发先进多目标检测系统的多功能平台.