Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Geometrically Well-Controlled Wireframe RNA Nanostructures With Bundled-Helix Edges.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

RNA nanostructures based on three-letter coding with non-canonical base pairs.

Nanoscale horizons·2026
Same author

Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Ligation-induced DNA self-assembly.

Nucleic acids research·2025
Same author

DNA-programmed responsive microorganism assembly with controlled patterns and behaviors.

Science advances·2025
Same author

Automated design of scaffold-free DNA wireframe nanostructures.

Nature communications·2025

相关实验视频

Updated: May 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K

在低温下以糖醇辅助的DNA自组合

Zhengyang Sun1,2, Wen Wang3,4,5, Bryan Wei1,2

  • 1School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Small (Weinheim an der Bergstrasse, Germany)
|September 1, 2025
PubMed
概括

研究人员使用糖醇开发了一种用于DNA纳米结构组装的新方法,在低温下实现自我组装. 这种技术与生物分子兼容,为分子和细胞生物学应用开辟了新的途径.

关键词:
DNA纳米结构葡萄糖自动组装

更多相关视频

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.5K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K

相关实验视频

Last Updated: May 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.5K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K

科学领域:

  • 生物技术
  • 纳米技术
  • 分子生物学

背景情况:

  • 热化是DNA纳米结构组装的标准,但需要高温,不适合生物分子应用.
  • 为了将DNA纳米结构与生物系统相结合,最好采用生理或较低的温度.

研究的目的:

  • 开发一种在任意温度,包括结条件下进行DNA自组合的新方法.
  • 评估这种新方法与自然生物分子的兼容性.

主要方法:

  • 在DNA自组反应系统中补充糖.
  • 在各种温度下进行DNA纳米结构的生产,包括零度以下的水平.
  • 使用细胞溶解物中的蛋白质进行联合组装试验.

主要成果:

  • 在任意温度下使用甘油成功生成多种DNA纳米结构.
  • 实现了前所未有的低温DNA纳米结构组合,
  • 证明基于糖的DNA纳米结构与功能良好的客蛋白完全兼容.

结论:

  • 在低温,任意温度下实现有效的DNA纳米结构自组.
  • 该方法与自然生物分子兼容,可用于分子和细胞生物学.
  • 这种方法为在生物环境中设计和利用DNA纳米结构提供了新的机会.