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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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相关实验视频

Updated: May 7, 2026

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

针对哺乳动物细胞和图案测序流细胞的优化ChIP-exo

Daniela Q James¹, Sohini Mukherjee¹, C Caiden Cannon¹

¹Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.

bioRxiv : the preprint server for biology

|September 2, 2025

概括

我们提出了一种精简的哺乳动物优化ChIP-exo (MO-ChIP-exo) 协议,用于高分辨率的全基因组蛋白质-DNA相互作用映射. 这种优化的方法克服了以前的技术障碍,提高了哺乳动物细胞研究的可访问性.

关键词:

在CTCF ChIP-exo 的使用情况没有NGS 在2000年染色体染色体免疫沉超声波测试

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Published on: February 24, 2021

相关实验视频

Last Updated: May 7, 2026

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

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Published on: April 30, 2019

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Published on: February 24, 2021

科学领域:

分子生物学
基因组学
表观遗传学

背景情况:

染色体免疫沉 (ChIP) 与外核酶消化 (ChIP-exo) 结合,提供全基因组的高分辨率蛋白质-DNA相互作用分析.
由于长时间的协议,定制试剂和测序平台的不兼容性,ChIP-exo的广泛采用受到限制.

研究的目的:

为哺乳动物细胞和当前测序技术优化和调整ChIP-exo库建设协议.
开发一个强大而高效的哺乳动物优化ChIP-exo (MO-ChIP-exo) 协议.

主要方法:

系统优化交叉链接,细胞采集和DNA库建设步骤.
对悬浮 (K562) 和附着 (HepG2,mESC) 哺乳动物细胞系的协议的调整.
通过与现有的ChIP-exo协议进行比较来验证.

主要成果:

通过MO-ChIP-exo协议,可以显著地简化图书馆的准备工作.
在各种哺乳动物细胞类型中表现出适应性和效率.
成功生成适合当前测序平台的高质量ChIP-exo库.

结论:

MO-ChIP-exo协议有效地解决了与标准ChIP-exo相关的技术挑战.
为哺乳动物系统中蛋白质-DNA相互作用的高分辨率绘制提供了更容易获得,更强大,更有效的方法.
促进ChIP-exo在基因组和表观基因组研究中的更广泛应用.