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相关实验视频

Updated: Jan 6, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

针对哺乳动物细胞和图案测序流细胞的优化ChIP-exo.

Daniela Q James¹, Sohini Mukherjee¹, C Caiden Cannon¹

¹Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United States.

G3 (Bethesda, Md.)

|November 9, 2025

概括

我们为哺乳动物细胞优化了ChIP-exo协议,创造了一种高分辨率全基因组蛋白质-DNA相互作用分析的简化方法. 这个新协议 (MO-ChIP-exo) 克服了以前的技术障碍,提高了研究人员的可访问性.

关键词:

在CTCF中,CTCF是指CTCF.在 ChIP-exo.没有NGS,没有NGS.下一个Seq 2000年在这种情况下,染色染色素染色素免疫沉的免疫沉.协议协议协议协议超声波的使用方法

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相关实验视频

Last Updated: Jan 6, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Published on: January 7, 2020

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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科学领域:

分子生物学分子生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

染色体免疫沉与外核酶 (ChIP-exo) 提供高分辨率的全基因组蛋白质-DNA相互作用映射.
广泛采用ChIP-exo受限于冗长的协议,自定义反应和测序平台的不兼容性.

研究的目的:

为哺乳动物细胞和现代测序技术优化和调整ChIP-exo库建设协议.
从哺乳动物样本开发一种更强大,更有效的方法来生成高质量的ChIP-exo库.

主要方法:

系统地优化交叉连接,细胞采集和库建设步骤,用于ChIP-exo.
对悬浮 (K562) 和附着 (HepG2,mESC) 哺乳动物细胞系进行协议的调整.
通过与现有的ChIP-exo协议进行比较来验证.

主要成果:

引入一个针对哺乳动物优化的ChIP-exo (MO-ChIP-exo) 协议.
在不同的哺乳动物细胞类型中证明了MO-ChIP-exo的适应性.
通过使用优化的协议,成功生成了高质量的ChIP-exo库.

结论:

MO-ChIP-exo协议有效地解决了与以前的ChIP-exo方法相关的技术挑战.
这种优化的协议提高了哺乳动物系统中蛋白质-DNA相互作用映射的效率和稳定性.
MO-ChIP-exo促进了在生物研究中更广泛地应用高分辨率ChIP-exo分析.